polX

polX
168

DNA polymerase X, involved in DNA repair, generates adaptive mutations by error-prone processing of A/P sites

locus
BSU_28590
Molecular weight
63.95 kDa
pI
5.31
Protein length
Gene length
function
DNA repair
product
DNA polymerase X
essential
no
ec
2.7.7.7
synonyms
polX, yshC

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1796 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,923,314 → 2,925,026
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The protein
Catalyzed reaction/ biological activity
template-dependent DNA polymerase, fills single nucleotide gaps [Pubmed|18938175]
has intrinsic 3'-5' exonuclease activity for resecting unannealed 3'-termini in gapped DNA substrates [Pubmed|18776221]
has intrinsic apurinic/apyrimidinic (AP) endonuclease activity [Pubmed|20974932]
2'-deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)
Protein family
N-terminal part: DNA polymerase type-X family (single member, according to UniProt)
C-terminal part: PHP family (single member, according to UniProt)
[wiki|Domains]
C-terminal PHP domain with an active site formed by nine histidines and aspartates that catalyzes 3'-5' exonuclease, AP-endonuclease, 3'-phosphodiesterase and 3'-phosphatase activities [pubmed|31289311]
Structure
[PDB|3AU2] (from Thermus thermophilus, complex with dGTP, 37% identity) [pubmed|22306405]
[AF|P94544]
Expression and Regulation
Operons
genes
[gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA]-[gene|93726C423313C653C4F71CB107FA12F686959E41|yshB]-[gene|D3954F2BCF6CE66505E4F48FCB4A61EE8D24F6D3|polX]-[gene|F8E82D9AE514B31892FE4530532691274ADA682B|rqcU]-[gene|18CEF3F1EE2E95A009699DE370CBB44F025B7B40|yshE]
description
[Pubmed|22383849]
regulation
constitutively expressed [Pubmed|23701187]
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[gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA]→[gene|18CEF3F1EE2E95A009699DE370CBB44F025B7B40|yshE]

2024-03-11 14:49:55

Jstuelk

80

a60f402276d016cd1954cecdf421cd68dd4fd667

C40CD769E454F6213CFF839AD02CA2F4B6A46C6B

Biological materials
Mutant
GP3560 (Δ([gene|D3954F2BCF6CE66505E4F48FCB4A61EE8D24F6D3|polX]-[gene|F8E82D9AE514B31892FE4530532691274ADA682B|rqcU])::kan), available in [wiki|Jörg Stülke]'s lab
MGNA-B003 (yshC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1002 NBRP B. subtilis, Japan]
BKE28590 (Δ[gene|D3954F2BCF6CE66505E4F48FCB4A61EE8D24F6D3|polX]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28590 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCATAACCCCCAGACG,  downstream forward: _UP4_TAAGTAAGGAGGCTCACACA
BKK28590 (Δ[gene|D3954F2BCF6CE66505E4F48FCB4A61EE8D24F6D3|polX]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28590 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCATAACCCCCAGACG,  downstream forward: _UP4_TAAGTAAGGAGGCTCACACA
labs
[wiki|Margarita Salas], Madrid, Spain [http://www.cbm.uam.es/mkfactory.esdomain/webs/CBMSO/plt_LineasInvestigacion.aspx?IdObjeto=28 link]
References
Reviews
22933559
Original Publications
20974932,18938175,18776221,22844091,24914186,28254425,22306405,31289311

D3954F2BCF6CE66505E4F48FCB4A61EE8D24F6D3

Page visits: 2467

Time of last update: 2024-03-28 16:36:54

Author of last update: MBenda