pncA

168

nicotinamidase

locus: BSU_31760

Molecular weight: 20.38 kDa

pI: 5.6

Protein length: 183 aa

Gene length: 552 bp

function: NAD salvage

product: nicotinamidase

essential: no

synonyms: pncA, yueJ

List of homologs in different organisms, belongs to COG1335 (Galperin et al., 2021)

Gene

Coordinates: 3,260,891  3,261,442

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The protein

Catalyzed reaction/ biological activity

• deamination of nicotinamide to nicotinic acid [pubmed|30107912]

Protein family

• [wiki|Isochorismatase family] (according to UniProt)

Structure

• [PDB|5ZN8]
• [PDB|6A8L]
• [AF|O32091]

Expression and Regulation

Operons

• genes: [gene|26430DCCEC263E7D03A3BBA4E7D636A250C3208B|pncA]-[gene|7CA7ADDBDDE46B0E6522704FD19C0BCF6FA9E058|pncB]

  description: [Pubmed|22383849]

• genes: [gene|17F2619E74A1604E86ED94E1BEE683D49E437A6C|yueH]-[gene|3918D82756A64E86E96A21ACEB34B0CF992D662D|yueI]-[gene|26430DCCEC263E7D03A3BBA4E7D636A250C3208B|pncA]-[gene|7CA7ADDBDDE46B0E6522704FD19C0BCF6FA9E058|pncB]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B563 (yueJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1562 NBRP B. subtilis, Japan]
• BKE31760 ([gene|26430DCCEC263E7D03A3BBA4E7D636A250C3208B|pncA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31760 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGCCGGCCTCCCCGCCA,  downstream forward: _UP4_GCAGAGTAAGGAAGGGGAAA
• BKK31760 ([gene|26430DCCEC263E7D03A3BBA4E7D636A250C3208B|pncA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31760 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGCCGGCCTCCCCGCCA,  downstream forward: _UP4_GCAGAGTAAGGAAGGGGAAA

References

Research papers

• 30107912