pghB

168

phage-derived gamma polyglutamate hydrolase

locus: BSU_12480

Molecular weight: 22.70 kDa

pI: 6.42

Protein length: 200 aa

Gene length: 603 bp

function: polyglutamate degradation

product: phage-derived gamma polyglutamic acid hydrolase

essential: no

synonyms: pghB, yjqB

List of homologs in different organisms, belongs to COG4195 (Galperin et al., 2021)

Gene

Coordinates: 1,319,011 → 1,319,613

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The protein

Catalyzed reaction/ biological activity

• hydrolysis of polyglutamic acid [Pubmed|26158264]

Protein family

• [wiki|UPF0714 family] (according to UniProt)

[wiki|Domains]

• DUF867 [http://pfam.xfam.org/family/PF05908 x], [[gamma-PGA hydrolase domain]] [Pubmed|26158264]

Structure

• [PDB|3A9L] (from B. subtilis phage NIT1, 41% identity) [pubmed|22105902]
• [AF|O34785]

Paralogous protein(s)

• [protein|DF95DED9A6E2160C151D7664C951B198EEA225E8|pghC], [protein|FD862CE6C4A37B17714F8F8687B3266D43EF5F63|pghL], [protein|8F9DCD77660706E4D25CD7875C014F6A2B5D8123|pghZ]

Expression and Regulation

Operons

• genes: [gene|BA7D1D112E934B68073DFC1F76248ED6157240E3|pghB]-[gene|59EC45E8731FE989152553A69ACEF6569E88F85C|yjqC]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B305 (yjqB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1304 NBRP B. subtilis, Japan]
• BKE12480 (Δ[gene|BA7D1D112E934B68073DFC1F76248ED6157240E3|pghB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12480 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTTGAACGCCCCCCTTT,  downstream forward: _UP4_TAAAACTAGACGGGACCTGC
• BKK12480 (Δ[gene|BA7D1D112E934B68073DFC1F76248ED6157240E3|pghB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12480 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTTGAACGCCCCCCTTT,  downstream forward: _UP4_TAAAACTAGACGGGACCTGC

References

• 26158264,22105902