pelB

168

pectate lyase

locus: BSU_18650

Molecular weight: 37.95 kDa

pI: 9.44

Protein length: 345 aa

Gene length: 1038 bp

function: degradation of polygalacturonic acid

product: pectate lyase

essential: no

ec: 4.2.2.10

synonyms: pelB, ppr

List of homologs in different organisms, belongs to COG3866 (Galperin et al., 2021)

Gene

Coordinates: 2,034,745  2,035,782

Advertisement

The protein

Catalyzed reaction/ biological activity

• Eliminative cleavage of (1->4)-alpha-D-galacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosyl groups at their non-reducing ends (according to UniProt)

Protein family

• [wiki|lyase 1 family] (according to UniProt)

Structure

• [PDB|3VMV] (from Bacillus sp. N165, 29% identity) [pubmed|22414692]
• [AF|O34819]

[wiki|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Biological materials

Mutant

• BKE18650 ([gene|19854503A5A0A19E72C8E1BEC65EECC243E96F79|pelB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18650 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCACAATATGCTTCCTTT,  downstream forward: _UP4_TGATATAAAAGAGGCCCTGC
• BKK18650 ([gene|19854503A5A0A19E72C8E1BEC65EECC243E96F79|pelB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18650 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCACAATATGCTTCCTTT,  downstream forward: _UP4_TGATATAAAAGAGGCCCTGC

References

• 18957862,22414692