papA

168

Xaa-Pro amino-peptidase

locus: BSU_24460

Molecular weight: 37.96 kDa

pI: 4.83

Protein length: 353 aa

Gene length: 1062 bp

function: degradation of proline-containing peptides

product: Xaa-Pro amino-peptidase

essential: no

ec: 3.4.11.9

synonyms: papA, yqhT

List of homologs in different organisms, belongs to COG0006 (Galperin et al., 2021)

Gene

Coordinates: 2,538,697  2,539,758

Phenotypes of a mutant

• inactivation of ''[gene|33886A1EAC13B684E92BF94201C46617AD2844B3|papA]'' reduces sporulation efficiency to 1.2% that of wild type cells; delayed entry into sporulation [Pubmed|26735940]

Advertisement

The protein

Protein family

• peptidase M24B family (with [protein|C95EEAC79B9E823BBBCF6B1960DABCCBB2AC03A4|papB], according to UniProt)

Structure

• [PDB|3Q6D] (the ''B. anthracis'' enzyme, 65% identity, 82% similarity)
• [AF|P54518]

Paralogous protein(s)

• [protein|C95EEAC79B9E823BBBCF6B1960DABCCBB2AC03A4|papB]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Mutant

• MGNA-C447 (yqhT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2445 NBRP B. subtilis, Japan]
• available in [wiki|Erhard Bremer]'s lab
• BKE24460 ([gene|33886A1EAC13B684E92BF94201C46617AD2844B3|papA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24460 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTTCATCTTGATTCTCCCCC,  downstream forward: _UP4_TGATTGGAATATAGGAGGAC
• BKK24460 ([gene|33886A1EAC13B684E92BF94201C46617AD2844B3|papA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24460 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTTCATCTTGATTCTCCCCC,  downstream forward: _UP4_TGATTGGAATATAGGAGGAC

Expression vectors

• pGP769: expression of Strep-''papA'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
• pGP1150: expression of ''papA''-Strep by [wiki|pGP382] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab

labs

• [wiki|Erhard Bremer], Marburg University, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi Homepage]

References

• 23144141,26735940