PWH844

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pWH844: click to enlarge
pWH844 cloning region: click to enlarge


vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein

the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

constructed in the Hillen lab

in E. coli: ampicillin resistance

similar plasmid: pGP570

sequencing primer:

  • JS39: 5’-TCTATCAACAGGAGTCCAAGC-3‘
  • SH71: 5‘-AACGGTGGTATATCCAGTG-3‘
  • PQE9-R348: 5’-TTGGGATATATCAACGGTGGTA-3’
  • JK37: 5’-GGATAACAATTATAATAGATTCAATTGTGAGCGGATAAC-3‘

F Schirmer, S Ehrt, W Hillen
Expression, inducer spectrum, domain structure, and function of MopR, the regulator of phenol degradation in Acinetobacter calcoaceticus NCIB8250.
J. Bacteriol.: 1997, 179(4);1329-36
[PubMed:9023219] [WorldCat.org] (P p)

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