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Vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein, after purification the tag can be cleaved off by thrombin

the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

constructed in the Stülke lab

based on pWH844

in E. coli: ampicillin resistance

similar plasmid: pWH844


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