PGP3273

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Vector for the construction of integrative (at the amyE site) expression in B. subtilis that can be induced by IPTG Does not replicate in B. subtilis; integrates into the amyE site


  • in E. coli: ampicillin resistance
  • in B. subtilis: chloramphenicol resistance
  • similar vectors: pAC5, pHT01, pGP3299: C-term. 6xHIS-tagged version of pHT01, pGP3232: N-term. STREP-tagged version of pHT01

sequencing primers:

  • CB242f: 5’-AGCTAACGGAAAAGGGAGCG-3’
  • LK49r: 5’-ATGAACTTTAATAAAATTGATTTAGACAATTGGAAGAGAAAAGAG-3‘

PGP3273.jpg