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The vector was constructed in Jörg Stülke's lab and it is suitable for fusion of C-terminal 3xFLAG-tag to the protein of interest in B. subtilis. The plasmid confers resistance to ampicillin and erythromycin in E. coli and B. subtilis, respectively. pGP1370 is based on the vector pBQ200. A Shine-Dalgarno sequence has to be fused to the open reading frame during PCR.

Sequencing primers:

  • M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
  • M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘
  • FX125 (rev; primes -85bp of MCS): 5‘-GGCTCGTATGTTGTGTGG-3‘
  • JN54 (fwd; primes +63bp of MCS): 5‘-GTGAAAAATGAGCCGAAAGCAG-3‘

The reference

Martin Lehnik-Habrink, Joseph Newman, Fabian M Rothe, Alexandra S Solovyova, Cecilia Rodrigues, Christina Herzberg, Fabian M Commichau, Richard J Lewis, Jörg Stülke
RNase Y in Bacillus subtilis: a Natively disordered protein that is the functional equivalent of RNase E from Escherichia coli.
J. Bacteriol.: 2011, 193(19);5431-41
[PubMed:21803996] [] [DOI] (I p)

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