PAC7

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pAC7: click to enlarge
pAC7 cloning region: click to enlarge


vector for the construction of translational lacZ fusions that can be integrated at the amyE site

does not replicate in B. subtilis, integrates into the amyE site

constructed in the Rapoport lab

in E. coli: ampicillin resistance

in B. subtilis: kanamycin resistance

similar vectors: pAC5, pAC6

sequencing primer:

  • PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
  • PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
  • Kancheckrev: 5‘-CTGCCTCCTCATCCTCTTCATCC-3‘

Y Weinrauch, T Msadek, F Kunst, D Dubnau
Sequence and properties of comQ, a new competence regulatory gene of Bacillus subtilis.
J. Bacteriol.: 1991, 173(18);5685-93
[PubMed:1715859] [WorldCat.org] (P p)

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