PAC6

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pAC6: click to enlarge

vector for the construction of transcriptional lacZ fusions that can be integrated at the amyE site

does not replicate in B. subtilis, integrates into the amyE site

constructed in the Rapoport lab

in E. coli: ampicillin resistance

in B. subtilis: chloramphenicol resistance

similar vectors: pAC5, pAC7

sequencing primer:

  • PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
  • PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
  • Catcheckrev: 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’

J Stülke, I Martin-Verstraete, M Zagorec, M Rose, A Klier, G Rapoport
Induction of the Bacillus subtilis ptsGHI operon by glucose is controlled by a novel antiterminator, GlcT.
Mol. Microbiol.: 1997, 25(1);65-78
[PubMed:11902727] [WorldCat.org] (P p)

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