nusB

168

transcription antitermination factor

locus: BSU_24320

Molecular weight: 14.74 kDa

pI: 4.76

Protein length: 131 aa

Gene length: 396 bp

function: stable RNA transcription

product: antitermination factor

essential: no

synonyms: nusB, yqhZ

List of homologs in different organisms, belongs to COG0781 (Galperin et al., 2021)

Gene

Coordinates: 2,529,267  2,529,662

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The protein

Protein family

• nusB family (single member, according to UniProt)

Structure

• [PDB|1EYV] (from Mycobacterium tuberculosis, 40% identity) [pubmed|10881194]
• [PDB|3R2C] (the [protein|6155468AFF216D97373CEF7B8C6E6C7BB9D4617F|nusB]-[protein|751D0B909922D4D931FF3B2285CF142D966FCD48|rpsJ] complex from Aquifex aeolicus) [pubmed|21652641]
• [AF|P54520]

Biological materials

Mutant

• MGNA-C368 (yqhZ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2366 NBRP B. subtilis, Japan]
• BKE24320 ([gene|6155468AFF216D97373CEF7B8C6E6C7BB9D4617F|nusB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24320 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTCTTTCTCCTTTGAT,  downstream forward: _UP4_TAGGAGGAAAGAAAATGACT
• BKK24320 ([gene|6155468AFF216D97373CEF7B8C6E6C7BB9D4617F|nusB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24320 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTCTTTCTCCTTTGAT,  downstream forward: _UP4_TAGGAGGAAAGAAAATGACT

References

• 36815589,11948165,16707701,11591660,2536750,10881194,21652641,30023707