nupN

168

lipoprotein, part of guanosine transporter

locus: BSU_31540

Molecular weight: 37.19 kDa

pI: 4.82

Protein length: 359 aa

Gene length: 1080 bp

function: uptake of guanosine

product: lipoprotein, part of guanosine transporter

essential: no

synonyms: nupN, yufN

List of homologs in different organisms, belongs to COG1744 (Galperin et al., 2021)

Gene

Coordinates: 3,239,930  3,241,009

Phenotypes of a mutant

• a ''[gene|19BA45BD691BBA4B23C4741C63DB729AA5D30FDA|nupN] [gene|C333F405F597FF260FFA0EC616B8CF6BF454815B|nupG]'' double mutant is unable to utilize externally provided guanosine [Pubmed|21926227]

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The protein

Protein family

• BMP lipoprotein family (with [protein|4DAB7847EA3848A34432C80124464543FA3DCA0F|med], according to UniProt)

Structure

• [PDB|2FQW] (from Treponema pallidum, 45% identity) [pubmed|16418175]
• [AF|O05252]

[wiki|Localization]

• cell membrane (according to UniProt),  membrane associated [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|19BA45BD691BBA4B23C4741C63DB729AA5D30FDA|nupN]-[gene|71546D1508EF17A208332E162CAE2C299987A569|nupO]-[gene|0BB235F189D30C9BE798560C31DA59FF0112CFD9|nupP]-[gene|7A80910F4E62BA15DE93FFC70E1908FB5CB9542D|nupQ]

  description: [Pubmed|21926227]

  regulation

  • repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455,21699902,21926227]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|12618455,21699902,21926227], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|21926227], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-B553 (yufN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1552 NBRP B. subtilis, Japan]
• BKE31540 ([gene|19BA45BD691BBA4B23C4741C63DB729AA5D30FDA|nupN]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGATATAACCCCCCAGAA,  downstream forward: _UP4_TAAGAATCGCAGGGATAAGG
• BKK31540 ([gene|19BA45BD691BBA4B23C4741C63DB729AA5D30FDA|nupN]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGATATAACCCCCCAGAA,  downstream forward: _UP4_TAAGAATCGCAGGGATAAGG

References

• 12618455,18763711,20935095,21699902,21926227,16418175