nasD

168

assimilatory nitrite reductase (subunit)

locus: BSU_03300

Molecular weight: 88.24 kDa

pI: 4.95

Protein length: 805 aa

Gene length: 2418 bp

function: utilization of nitrite as nitrogen source

product: assimilatory nitrite reductase (subunit)

essential: no

ec: 1.7.1.4

synonyms: nasD, nasBC, nirB

List of homologs in different organisms, belongs to COG1251 (Galperin et al., 2021)

Gene

Coordinates: 355,764 → 358,181

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The protein

Catalyzed reaction/ biological activity

• 2 H₂O + 3 NADP⁺ + NH₄⁺ --> 5 H⁺ + 3 NADPH + nitrite (according to UniProt)
• 2 H₂O + 3 NAD⁺ + NH₄⁺ --> 5 H⁺ + 3 NADH + nitrite (according to UniProt)

Protein family

• nitrite and sulfite reductase 4Fe-4S domain family (with [protein|A4C9E4D1163B703A917BFCF97A5F575B19EDDD8D|cysI], according to UniProt)

[wiki|Cofactors]

• Fe-S cluster  [Pubmed|11289299]
• FAD  [Pubmed|11289299]

Structure

• [PDB|3KLJ] (from Clostridium acetobutylicum, corresponds to aa 5 ... 399, 32% identity) [pubmed|20017214]
• [AF|P42435]

Paralogous protein(s)

• [protein|3F87227DC3371746B3823730B6A7024D348C151E|nasB]

Expression and Regulation

Operons

• genes: [gene|3F87227DC3371746B3823730B6A7024D348C151E|nasB]-[gene|EC8BF9A3BA88FE2A78C6F78A888E0D1E25C47BC5|nasC]-[gene|FAAC0F819AEC8E055CCEE86D93C8DE5584DC4B23|nasD]-[gene|A8998F46F68155A5C8AE2A2FBB95CA5A1635BB2B|nasE]-[gene|159A6D0D45B5F0F055228357FD9A3BF8E5A999E5|nasF]

  description: [Pubmed|7836289]

  regulation

  • ''[protein|search|nasD]'': expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]

  regulatory mechanism

  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]: activation, [Pubmed|10972836], in [regulon|protein:1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD regulon]
  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [PubMed|8799114,9765565], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
  • [protein|EC6697D5D945B7E5083AFED9218748763C443278|nsrR]: repression, [Pubmed|16885456], in [regulon|protein:EC6697D5D945B7E5083AFED9218748763C443278|nsrR regulon]
  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7836289], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

• genes: [gene|FAAC0F819AEC8E055CCEE86D93C8DE5584DC4B23|nasD]-[gene|A8998F46F68155A5C8AE2A2FBB95CA5A1635BB2B|nasE]-[gene|159A6D0D45B5F0F055228357FD9A3BF8E5A999E5|nasF]

  description: [Pubmed|9765565]

  regulation

  • ''[protein|search|nasD]'': expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]

Biological materials

Mutant

• 1A973 ( ''nasD''::''phleo''), [Pubmed|7868621], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A973&Search=1A973 BGSC]
• BKE03300 (Δ[gene|FAAC0F819AEC8E055CCEE86D93C8DE5584DC4B23|nasD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAGATGATCCGCTCCTT,  downstream forward: _UP4_TAATGACAAAAACTATCATT
• BKK03300 (Δ[gene|FAAC0F819AEC8E055CCEE86D93C8DE5584DC4B23|nasD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAGATGATCCGCTCCTT,  downstream forward: _UP4_TAATGACAAAAACTATCATT

References

Reviews

• 22103536,11289299

Original Publications

• 12823818,7868621,8799114,9765565,10972836,16885456,21091510,24214949,25755103,28439033,20017214,34898263