murAB

168

UDP-N-acetylglucosamine 1-carboxyvinyltransferase, required for efficient spore engulfment

locus: BSU_37100

Molecular weight: 45.85 kDa

pI: 5.83

Protein length: 429 aa

Gene length: 1290 bp

function: peptidoglycan precursor biosynthesis

product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase

essential: no

ec: 2.5.1.7

synonyms: murAB, murZ, lssF, rev-4

List of homologs in different organisms, belongs to COG0766 (Galperin et al., 2021)

Gene

Coordinates: 3,806,086  3,807,375

Advertisement

The protein

Catalyzed reaction/ biological activity

• phosphoenolpyruvate + UDP-N-acetyl-α-D-glucosamine --> phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-α-D-glucosamine(according to UniProt)

Protein family

• EPSP synthase family (with [protein|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA] and [protein|CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1|aroE], according to UniProt)

Structure

• [PDB|3SG1] (from ''B. anthracis'', 50% identity, 81% similarity)
• [AF|P19670]

Paralogous protein(s)

• [protein|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB]-[gene|0AFCE5C602B073B29DB15DB3CDD153E05E3D02BD|glpX]

  description: [Pubmed|19270101]

  regulation

  • constitutive [Pubmed|19270101]

Biological materials

Mutant

• BKE37100 ([gene|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37100 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATAGTCTCCTTCCATA,  downstream forward: _UP4_TAATGGTTTCTTGTGAGAGG
• BKK37100 ([gene|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37100 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATAGTCTCCTTCCATA,  downstream forward: _UP4_TAATGGTTTCTTGTGAGAGG

Expression vectors

• pGP2595: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab

References

Reviews

• 21822642

Original Publications

• 19270101,36066101,36815589