murAA

168

UDP-N-acetylglucosamine 1-carboxyvinyltransferase

locus: BSU_36760

Molecular weight: 46.54 kDa

pI: 5.45

Protein length: 436 aa

Gene length: 1311 bp

function: peptidoglycan precursor biosynthesis

product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase

essential: yes

ec: 2.5.1.7

synonyms: murAA, murA

List of homologs in different organisms, belongs to COG0766 (Galperin et al., 2021)

Gene

Coordinates: 3,777,949 → 3,779,259

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• phosphoenolpyruvate + UDP-N-acetyl-α-D-glucosamine --> phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-α-D-glucosamine (according to UniProt)

Protein family

• EPSP synthase family (with [protein|CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1|aroE] and [protein|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB], according to UniProt)

Structure

• [PDB|3SG1] (from ''B. anthracis'', 79% identity, 94% similarity)
• [AF|P70965]

Paralogous protein(s)

• [protein|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)
• division septa [pubmed|36649910]

Additional information

• [protein|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA] is subject to [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]-dependent degradation upon glucose starvation  [Pubmed|14763982,17981983]

Expression and Regulation

Operons

• genes: [gene|32CE4F77F08F926152BECAB56E2841E1FFB27600|ywzB]-[gene|3A8E0417C4EC85FDDE1178B775F6579C8F1009C6|spoIIT]-[gene|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]

  description: [Pubmed|22383849]

  regulation

  • expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [Pubmed|26735940]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|22383849], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|26735940], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]

• genes: [gene|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]

  description: [Pubmed|9353933]

  regulation

  • expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [Pubmed|26735940]

• genes: [gene|3A8E0417C4EC85FDDE1178B775F6579C8F1009C6|spoIIT]-[gene|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]

  description: [Pubmed|26735940]

  regulation

  • expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [Pubmed|26735940]

additional information

• the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]

Biological materials

Expression vectors

• pRSETA-murAA, Expression of His(6)-MurAA, avalable in [wiki|Ulf Gerth]'s lab [Pubmed|14763982]
• pGP2597: (IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab

Antibody

• avalable in [wiki|Ulf Gerth]'s lab [Pubmed|14763982]

References

Reviews

• 21822642

Original Publications

• 14763982,18063720,19899805,14763982,8994972,9485407,26735940,22383849,26883633,36649910,36815589