msrA

msrA
168

peptide methionine sulfoxide reductase

locus
BSU_21690
Molecular weight
20.04 kDa
pI
5.71
Protein length
Gene length
function
regeneration of methionine and restoration of protein function after oxidative damage
product
peptide methionine sulfoxide reductase
essential
no
ec
1.8.4.11
synonyms
msrA, yppP

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0225 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,287,529  2,288,062
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The protein
Catalyzed reaction/ biological activity
[thioredoxin]-disulfide + H2O + L-methionyl-[protein] --> [thioredoxin]-dithiol + L-methionyl-(S)-S-oxide-[protein](according to UniProt)
[thioredoxin]-disulfide + H2O + L-methionine --> [thioredoxin]-dithiol + L-methionine (S)-S-oxide (according to UniProt)
Protein family
MsrA Met sulfoxide reductase family (single member, according to UniProt)
Structure
[PDB|2GT3] (from E. coli, 45% identity) [pubmed|17157315]
[AF|P54154]
Expression and Regulation
Operons
genes
[gene|389245FDE13226D65DF117E28B5DA346DC856860|msrA]-[gene|BF8A3C916C4B673D30AEF7C330C9BD5A487AB0EA|msrB]
description
[Pubmed|18662407]
regulation
induced by oxidative stress ([protein|search|Spx])
regulatory mechanism
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, oxidized [protein|2C6386E9A63F410558D168798D077DF91590F454|spx] binds [protein|5996A5C25E108A3C4562686BF34A59CB14FD56EB|rpoA] and enhances [protein|389245FDE13226D65DF117E28B5DA346DC856860|msrA]-[protein|BF8A3C916C4B673D30AEF7C330C9BD5A487AB0EA|msrB] operon transcription [Pubmed|18662407], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|18662407,17289755], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|389245FDE13226D65DF117E28B5DA346DC856860|msrA]→[gene|BF8A3C916C4B673D30AEF7C330C9BD5A487AB0EA|msrB]

2024-03-10 08:55:35

ghost

45

260530b389542edd9065dae32bb081f8ff5b9af0

31A91806DA37296DA2E76252C321BA878BFF3D0A

Biological materials
Mutant
MGNA-A894 (yppP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/894 NBRP B. subtilis, Japan]
BKE21690 ([gene|389245FDE13226D65DF117E28B5DA346DC856860|msrA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE21690 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACTGGTGCCTCCTTTGT,  downstream forward: _UP4_TAAATGGCGTACAATAAAGA
BKK21690 ([gene|389245FDE13226D65DF117E28B5DA346DC856860|msrA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK21690 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACTGGTGCCTCCTTTGT,  downstream forward: _UP4_TAAATGGCGTACAATAAAGA
References
18662407,17157315

389245FDE13226D65DF117E28B5DA346DC856860

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Time of last update: 2024-03-28 13:18:29

Author of last update: Melvin.boenninger