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  • Description: large conductance mechanosensitive channel protein, prevents selective release of cytoplasmic proteins in a hypotonic environment

Gene name mscL
Synonyms ywpC
Essential no
Product large conductance mechanosensitive channel protein
Function resistance to osmotic downshockglycine betaine export
Gene expression levels in SubtiExpress: mscL
Metabolic function and regulation of this protein in SubtiPathways:
MW, pI 14 kDa, 4.649
Gene length, protein length 390 bp, 130 aa
Immediate neighbours ywpD, ywpB
Sequences Protein DNA DNA_with_flanks
Genetic context
MscL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MscL expression.png


Categories containing this gene/protein

coping with hypo-osmotic stress, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU36360

Phenotypes of a mutant

sensitive to osmotic downshock (> 0.5 M) during logarithmic growth PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: mscL family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3HZQ (MscL of Staphylococcus aureus) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: expressed in logarithmic phase PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Jan Maarten van Dijl, Groningen, Netherlands

Erhard Bremer, University of Marburg, Germany homepage

Your additional remarks



Marcel Reuter, Nicholas J Hayward, Susan S Black, Samantha Miller, David T F Dryden, Ian R Booth
Mechanosensitive channels and bacterial cell wall integrity: does life end with a bang or a whimper?
J R Soc Interface: 2014, 11(91);20130850
[PubMed:24258154] [] [DOI] (I e)

Ian R Booth, Paul Blount
The MscS and MscL families of mechanosensitive channels act as microbial emergency release valves.
J. Bacteriol.: 2012, 194(18);4802-9
[PubMed:22685280] [] [DOI] (I p)

Ching Kung, Boris Martinac, Sergei Sukharev
Mechanosensitive channels in microbes.
Annu. Rev. Microbiol.: 2010, 64;313-29
[PubMed:20825352] [] [DOI] (I p)

Valeria Vásquez, Eduardo Perozo
Structural biology: A channel with a twist.
Nature: 2009, 461(7260);47-9
[PubMed:19727188] [] [DOI] (I p)

Christopher D Pivetti, Ming-Ren Yen, Samantha Miller, Wolfgang Busch, Yi-Hsiung Tseng, Ian R Booth, Milton H Saier
Two families of mechanosensitive channel proteins.
Microbiol. Mol. Biol. Rev.: 2003, 67(1);66-85, table of contents
[PubMed:12626684] [] (P p)

Original Publications

Thijs R H M Kouwen, Erik N Trip, Emma L Denham, Mark J J B Sibbald, Jean-Yves F Dubois, Jan Maarten van Dijl
The large mechanosensitive channel MscL determines bacterial susceptibility to the bacteriocin sublancin 168.
Antimicrob. Agents Chemother.: 2009, 53(11);4702-11
[PubMed:19738010] [] [DOI] (I p)

Zhenfeng Liu, Chris S Gandhi, Douglas C Rees
Structure of a tetrameric MscL in an expanded intermediate state.
Nature: 2009, 461(7260);120-4
[PubMed:19701184] [] [DOI] (I p)

Paul G Wahome, Ann E Cowan, Barbara Setlow, Peter Setlow
Levels and localization of mechanosensitive channel proteins in Bacillus subtilis.
Arch. Microbiol.: 2009, 191(5);403-14
[PubMed:19252899] [] [DOI] (I p)

Thijs R H M Kouwen, Haike Antelmann, René van der Ploeg, Emma L Denham, Michael Hecker, Jan Maarten van Dijl
MscL of Bacillus subtilis prevents selective release of cytoplasmic proteins in a hypotonic environment.
Proteomics: 2009, 9(4);1033-43
[PubMed:19160392] [] [DOI] (I p)

Tamara Hoffmann, Clara Boiangiu, Susanne Moses, Erhard Bremer
Responses of Bacillus subtilis to hypotonic challenges: physiological contributions of mechanosensitive channels to cellular survival.
Appl. Environ. Microbiol.: 2008, 74(8);2454-60
[PubMed:18310427] [] [DOI] (I p)

Paul G Wahome, Peter Setlow
Growth, osmotic downshock resistance and differentiation of Bacillus subtilis strains lacking mechanosensitive channels.
Arch. Microbiol.: 2008, 189(1);49-58
[PubMed:17665170] [] [DOI] (P p)

Paul G Wahome, Peter Setlow
The synthesis and role of the mechanosensitive channel of large conductance in growth and differentiation of Bacillus subtilis.
Arch. Microbiol.: 2006, 186(5);377-83
[PubMed:16897034] [] [DOI] (P p)

P C Moe, P Blount, C Kung
Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation.
Mol. Microbiol.: 1998, 28(3);583-92
[PubMed:9632260] [] (P p)

E Presecan, I Moszer, L Boursier, H C Cruz Ramos, V de la Fuente, M F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading, Engl.): 1997, 143 ( Pt 10);3313-28
[PubMed:9353933] [] (P p)

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