mntH

168

manganese transporter (proton symport)

locus: BSU_04360

Molecular weight: 45.52 kDa

pI: 9.89

Protein length: 425 aa

Gene length: 1278 bp

function: manganese uptake

product: manganese transporter (proton symport))

essential: no

synonyms: mntH, ydaR

List of homologs in different organisms, belongs to COG1914 (Galperin et al., 2021)

Gene

Coordinates: 491,147  492,424

Phenotypes of a mutant

• suppresses the Mn²⁺ sensitivity of a [gene|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR] mutant [pubmed|31964700]

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The protein

Catalyzed reaction/ biological activity

• uptake of Mn²⁺

Protein family

• NRAMP family (with [protein|9AD419E3CB86C54A0F11E96C82E80A357C6B23D2|ycsG], according to UniProt)

Structure

• [PDB|4WGV] (from ''Staphylococcus capitis'', 40% identity) [Pubmed|25326704]
• [AF|P96593]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|353DADE8E57A0F1895CCEB62701F9273EEB5EB45|mntH]

  description: [Pubmed|10760146]

  regulation

  • repressed at high Mn(II) concentrations (MntR) [Pubmed|10760146]

  regulatory mechanism

  • [protein|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR]: repression, [Pubmed|10760146], in [regulon|protein:FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR regulon]

Biological materials

Mutant

• MGNA-C116 (ydaR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2114 NBRP B. subtilis, Japan]
• JH642 and its derivatives: natural deletion of the 18 kb [gene|467E9CF20B545A976614968E7DD5497F9390D344|ydzA]-[gene|353DADE8E57A0F1895CCEB62701F9273EEB5EB45|mntH] region encompassing the genes [gene|467E9CF20B545A976614968E7DD5497F9390D344|ydzA]-[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]-[gene|69DEE29383E40F49890CC8314DFBF56D70D35113|ydaJ]-[gene|70ED3CE683F3A3F785480F40986CABE7729C17C7|ydaK]-[gene|28D80C5497AE29A24AD6B9A1A713A2B3915F2CED|ydaL]-[gene|961538BEDD27C24FC073AFB7AB3268E3D46783CC|ydaM]-[gene|ADE0122D8FFF2F78B6D21031B7D02A323A13ED63|ydaN]-[gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA]-[gene|6085BDAD40F719555EA4EEA7BBAD74103BD03D0F|mutT]-[gene|2883FC60CD69948D5C43BDDFEFBE7A1CA7C743C7|ydaP]-[gene|87834D5C47064BEFF26F086A8202C311F28F9D38|ydzK]-[gene|353DADE8E57A0F1895CCEB62701F9273EEB5EB45|mntH] [pubmed|18670626]
• BKE04360 ([gene|353DADE8E57A0F1895CCEB62701F9273EEB5EB45|mntH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCTCACCTGAATTCTG,  downstream forward: _UP4_TAAAAAAACCGGCTTCTAAA
• BKK04360 ([gene|353DADE8E57A0F1895CCEB62701F9273EEB5EB45|mntH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCTCACCTGAATTCTG,  downstream forward: _UP4_TAAAAAAACCGGCTTCTAAA

labs

• [wiki|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]

References

Reviews

• 36313567,34037759

Original Publications

• 10760146,12950915,10760146,25326704,31964700