mmgB

168

3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)

locus: BSU_24160

Molecular weight: 26.92 kDa

pI: 6.6

Protein length: 287 aa

Gene length: 864 bp

function: mother cell metabolism

product: 3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)

essential: no

ec: 1.1.1.157

synonyms: mmgB, yqiM

List of homologs in different organisms, belongs to COG1250 (Galperin et al., 2021)

Gene

Coordinates: 2,511,973 → 2,512,836

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The protein

Catalyzed reaction/ biological activity

• (3S)-hydroxybutanoyl-CoA + NADP⁺ --> acetoacetyl-CoA + H⁺ + NADPH (according to UniProt)

Protein family

• 3-hydroxyacyl-CoA dehydrogenase family (with [protein|4787161A022E938A6D58A505D92369F889C7DE04|fadN], according to UniProt)

Structure

• [PDB|4R1N] (from Clostridium butyricum, 56% identity) [pubmed|25112316]
• [AF|P45856]

Biological materials

Mutant

• BKE24160 (Δ[gene|FB95ABDC8557F26525E8B211595C130A7203ABC0|mmgB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24160 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCAAATCCCCTTTTTCA,  downstream forward: _UP4_TGAAGTCTATTCCATCCGGG
• BKK24160 (Δ[gene|FB95ABDC8557F26525E8B211595C130A7203ABC0|mmgB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24160 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCAAATCCCCTTTTTCA,  downstream forward: _UP4_TGAAGTCTATTCCATCCGGG

References

• 8759838,15699190,25112316,30792386