MccB

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  • Description: cystathionine lyase/ homocysteine gamma-lyase

Gene name mccB
Synonyms yrhB
Essential no
Product cystathionine lyase/ homocysteine gamma-lyase
Function methionine-to-cysteine conversion
Gene expression levels in SubtiExpress: mccB
Metabolic function and regulation of this protein in SubtiPathways:
mccB
MW, pI 40 kDa, 5.215
Gene length, protein length 1137 bp, 379 aa
Immediate neighbours yrhC, mccA
Sequences Protein DNA DNA_with_flanks
Genetic context
YrhB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MccB expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, membrane proteins

This gene is a member of the following regulons

CymR regulon, Spx regulon

The gene

Basic information

  • Locus tag: BSU27250

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-cystathionine + H2O = L-cysteine + NH3 + 2-oxobutanoate (according to Swiss-Prot)
  • Protein family: trans-sulfuration enzymes family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed in the presence of cysteine (CymR) PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 164 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Marie-Françoise Hullo, Sandrine Auger, Olga Soutourina, Octavian Barzu, Mireille Yvon, Antoine Danchin, Isabelle Martin-Verstraete
Conversion of methionine to cysteine in Bacillus subtilis and its regulation.
J. Bacteriol.: 2007, 189(1);187-97
[PubMed:17056751] [WorldCat.org] [DOI] (P p)

Soon-Yong Choi, Dindo Reyes, Montira Leelakriangsak, Peter Zuber
The global regulator Spx functions in the control of organosulfur metabolism in Bacillus subtilis.
J. Bacteriol.: 2006, 188(16);5741-51
[PubMed:16885442] [WorldCat.org] [DOI] (P p)

Sergine Even, Pierre Burguière, Sandrine Auger, Olga Soutourina, Antoine Danchin, Isabelle Martin-Verstraete
Global control of cysteine metabolism by CymR in Bacillus subtilis.
J. Bacteriol.: 2006, 188(6);2184-97
[PubMed:16513748] [WorldCat.org] [DOI] (P p)

Shunji Nakano, Michiko M Nakano, Ying Zhang, Montira Leelakriangsak, Peter Zuber
A regulatory protein that interferes with activator-stimulated transcription in bacteria.
Proc. Natl. Acad. Sci. U.S.A.: 2003, 100(7);4233-8
[PubMed:12642660] [WorldCat.org] [DOI] (P p)