lonB

168

Lon-like ATP-dependent protease

locus: BSU_28210

Molecular weight: 60.26 kDa

pI: 6.27

Protein length: 552 aa

Gene length: 1659 bp

function: protein quality control

product: Lon-like ATP-dependent protease

essential: no

synonyms: lonB, ysxF

List of homologs in different organisms, belongs to COG1067 (Galperin et al., 2021)

Gene

Coordinates: 2,882,971 → 2,884,629

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The protein

Catalyzed reaction/ biological activity

• Hydrolysis of proteins in presence of ATP (according to UniProt)

Protein family

• Peptidase S16 family (with [protein|1A5C1BDE844AA56B90C0E4A02D74978676487E99|ddcP] and [protein|FF15BE26BCC78EC1301C58A51AF0A519D7BE9ADC|lonA], according to UniProt)

[wiki|Domains]

• Lon proteolytic (aa 349-535) (according to UniProt)

Structure

• [PDB|6U5Z] (from E. coli, corresponds to aa 93 ... 538, 27% identity)
• [AF|P42425]

[wiki|Localization]

• localized to the forespore membrane early in development, followed by localization throughout the forespore later in development [Pubmed|18689473]

Expression and Regulation

Operons

• genes: [gene|CA90AAE5320C20F93F5ED0F167294C28495756FF|lonB]

  description: [Pubmed|9852015]

  regulation

  • expressed early during sporulation in the forespore ([protein|search|SigF]) [Pubmed|16497325,11325926]

  sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|16497325,11325926], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]

Biological materials

Mutant

• BKE28210 (Δ[gene|CA90AAE5320C20F93F5ED0F167294C28495756FF|lonB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGTGGTCCCTCCTGAAA,  downstream forward: _UP4_TAAACCCTATTTTGATAATA
• BKK28210 (Δ[gene|CA90AAE5320C20F93F5ED0F167294C28495756FF|lonB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGTGGTCCCTCCTGAAA,  downstream forward: _UP4_TAAACCCTATTTTGATAATA

References

Reviews

• 23479438,34410368

Original Publications

• 16497325,11325926,9852015,18689473,34757797,36232729