lipL

168

N-amidotransferase, transfers lipoic acid from lipoyl-[protein|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH] to [protein|C4B6C3EE560C8BD353FEABB1A607C89C20DC8D34|acoC], [protein|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB], [protein|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB], and [protein|2F40086E35FA32136B9A89C530A86D714FE9460C|pdhC], and from lipoyl-[protein|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB] to [protein|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB] and [protein|2F40086E35FA32136B9A89C530A86D714FE9460C|pdhC]

locus: BSU_37640

Molecular weight: 31.27 kDa

pI: 5.27

Protein length: 281 aa

Gene length: 846 bp

function: lipoic acid metabolism

product: protein N-octanoyltransferase

essential: no

ec: 2.3.1.204

synonyms: lipL, ipa-90d, ywfL

List of homologs in different organisms, belongs to COG0095 (Galperin et al., 2021)

Gene

Coordinates: 3,863,415 → 3,864,260

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The protein

Catalyzed reaction/ biological activity

• transfers lipoic acid from lipoyl-[protein|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH] to [protein|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB], [protein|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB], and [protein|2F40086E35FA32136B9A89C530A86D714FE9460C|pdhC], and from lipoyl-[protein|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB] to [protein|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB] and [protein|2F40086E35FA32136B9A89C530A86D714FE9460C|pdhC] [pubmed|31066113]
• [glycine-cleavage complex H protein]-N⁶-octanoyl-L-lysine + [lipoyl-carrier protein]-L-lysine --> [glycine-cleavage complex H protein]-L-lysine + [lipoyl-carrier protein]-N⁶-octanoyl-L-lysine (according to UniProt)

Protein family

• Octanoyltransferase LipL family (single member, according to UniProt)

[wiki|Domains]

• [wiki|BPL/LPL catalytic domain] (aa 45-253) (according to UniProt)

Structure

• [PDB|2P5I] (from ''Bacillus halodurans'', 56% identity)
• [AF|P39648]

Expression and Regulation

Operons

• genes: [gene|F2E881F4BE0968C75BD75A5EFF4C7CA642034796|lipL]

  description: [Pubmed|9353933]

Biological materials

Mutant

• MGNA-B667 (ywfL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1666 NBRP B. subtilis, Japan]
• BKE37640 (Δ[gene|F2E881F4BE0968C75BD75A5EFF4C7CA642034796|lipL]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37640 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTATGTAAAACCCTTTC,  downstream forward: _UP4_TGAACGTTCTTCGCATGCCG
• BKK37640 (Δ[gene|F2E881F4BE0968C75BD75A5EFF4C7CA642034796|lipL]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37640 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTATGTAAAACCCTTTC,  downstream forward: _UP4_TGAACGTTCTTCGCATGCCG

References

Reviews

• 27074917

Original Publications

• 9353933,21338420,21338421,31066113