kdgT

168

2-keto-3-deoxygluconate permease

locus: BSU_22090

Molecular weight: 34.16 kDa

pI: 8.94

Protein length: 330 aa

Gene length: 993 bp

function: utilization of galacturonic acid

product: 2-keto-3-deoxygluconate permease

essential: no

synonyms: kdgT

List of homologs in different organisms

Gene

Coordinates: 2,321,972 → 2,322,964

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The protein

Protein family

• KdgT transporter family (single member, according to UniProt)

Structure

• [AF|P50847]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|891A32A19D1353BA566FF121BBCA7B986D31D129|kdgR]-[gene|8A0BB2BE0AAAA7FF834A4549E8E2AF343FB10571|kdgK]-[gene|826BFCE6ED2113BB0510E798C6CDB05C4665E0E2|kdgA]-[gene|EB6F40F8651654E0AB09447C5AD670C35A59F261|kdgT]

  description: [Pubmed|9846747]

  regulation

  • induced by galacturonate ([protein|891A32A19D1353BA566FF121BBCA7B986D31D129|kdgR]) [Pubmed|9846747]

  regulatory mechanism

  • [protein|891A32A19D1353BA566FF121BBCA7B986D31D129|kdgR]: repression, [Pubmed|9846747], in [regulon|protein:891A32A19D1353BA566FF121BBCA7B986D31D129|kdgR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|9846747], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9846747], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

additional information

• [protein|search|translation] is likely to require [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]

Biological materials

Mutant

• BKE22090 (Δ[gene|EB6F40F8651654E0AB09447C5AD670C35A59F261|kdgT]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22090 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTGTGGATTCTCCCAT,  downstream forward: _UP4_TGAATAAAAAGGCTGCCACA
• BKK22090 (Δ[gene|EB6F40F8651654E0AB09447C5AD670C35A59F261|kdgT]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22090 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTGTGGATTCTCCCAT,  downstream forward: _UP4_TGAATAAAAAGGCTGCCACA

References

• 9846747