iolG

168

inositol 2-dehydrogenase

locus: BSU_39700

Molecular weight: 38.20 kDa

pI: 4.86

Protein length: 344 aa

Gene length: 1035 bp

function: myo-inositol catabolism

product: inositol 2-dehydrogenase

essential: no

ec: 1.1.1.18

synonyms: iolG, idh, iol

List of homologs in different organisms, belongs to COG0673 (Galperin et al., 2021)

Gene

Coordinates: 4,075,785  4,076,819

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The protein

Catalyzed reaction/ biological activity

• myo-inositol + NAD⁺ --> H⁺ + NADH + scyllo-inosose (according to UniProt)
• 1D-chiro-inositol + NAD⁺ --> H⁺ + NADH + scyllo-inosine (according to UniProt)

Protein family

• [wiki|Gfo/Idh/MocA family] (according to UniProt)

[wiki|Cofactors]

• NAD⁺ [Pubmed|20809899]

Structure

• [PDB|3MZ0] [Pubmed|20809899]
• [AF|P26935]

Paralogous protein(s)

• [protein|942BAE9FA023DFCA06B6D26A856A72F2979E23FA|yrbE], [protein|AA7DCEFB17FD6F9C4F65235E9A247A6EC2242B06|iolX], [protein|03BE6DA854612B3F6741E0E167AB310F0DE96285|yfiI], [protein|29DAF5343A42EAD8DB97925FE7D049410F7B7FCA|ntdC], [protein|484D17480DFAD3667E0EBC6D38854A7546A8DB46|yteT], [protein|7DFE74C751A67CCC84579D52005E1399B0A10166|iolW], [protein|920C5750CB95102B073C32874D512E8D636D2C7D|iolU]

Expression and Regulation

Operons

• genes: [gene|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA]-[gene|0903CCBE053230998A9123F3269868E952C0F76B|iolB]-[gene|AAE60753464EAA32379521531FDCAF64534D5F40|iolC]-[gene|AA79B6F039EC5B79F4419A5F358AE5AE8292E731|iolD]-[gene|C31AF6C8050D453D9011B0793403791F1851BA69|iolE]-[gene|14813DE6C6DF7AB031BA05AED7D31AE9E8F8498E|iolF]-[gene|93C1DF93CAFC5AE726523A91A3BA7470017FF6DC|iolG]-[gene|FD64E9D00BB03B510C7F7CA3935558A2195532A9|iolH]-[gene|2742FCE60AA87B2BE5A0B5C34F617E10DDE7F126|iolI]-[gene|1EFB5C8F28146D1EA3B615F548D0CF262D2B4DF5|iolJ]

  description: [Pubmed|9226270]

  regulation

  • induced by inositol ([protein|search|IolR]) [Pubmed|9887260,9226270]

  regulatory mechanism

  • [protein|5F4ABBC8ECAD6F6C10EC39B2B0F0BC73F11522CE|iolR]: repression, [Pubmed|9887260,9226270], in [regulon|protein:5F4ABBC8ECAD6F6C10EC39B2B0F0BC73F11522CE|iolR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|10666464], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9226270], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-B698 (iolG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1697 NBRP B. subtilis, Japan]
• BKE39700 ([gene|93C1DF93CAFC5AE726523A91A3BA7470017FF6DC|iolG]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39700 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGACAGCCACTCCTTCT,  downstream forward: _UP4_AACTAAAAAACAGAGGAGTG
• BKK39700 ([gene|93C1DF93CAFC5AE726523A91A3BA7470017FF6DC|iolG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39700 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGACAGCCACTCCTTCT,  downstream forward: _UP4_AACTAAAAAACAGAGGAGTG

labs

• [wiki|Yasutaro Fujita], University of Fukuyama, Japan
• [[Ken-ichi Yoshida]], Kobe University, Japan

References

• 9226270,6322857,1761221,112095,9887260,18310071,112095,20809899,23952058,24325193,25019153