int

168

integrase

locus: BSU_04800

Molecular weight: 43.32 kDa

pI: 9.76

Protein length: 368 aa

Gene length: 1107 bp

function: excision of the conjugative transposon ICEBs1 from the trnS-leu2 locus

product: integrase

essential: no

synonyms: int, ydcL

List of homologs in different organisms, belongs to COG4974 (Galperin et al., 2021)

Gene

Coordinates: 529,505  530,611

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The protein

Protein family

• [wiki|phage integrase family] (according to UniProt)

Structure

• [PDB|4A8E] (from Pyrococcus abyssi, 25% identity) [pubmed|23667562]
• [AF|P96629]

Expression and Regulation

Operons

• genes: [gene|DD1C8F3A4809785BD6A6047D39B42AB2C605E161|immR]-[gene|1599BBAC05EB62A847F73572CD777BC472301DE8|immA]-[gene|47AE982C5E71F31B5AF04C4F50593C2629F8EDAF|int]

  description: [pubmed|22383849]

Biological materials

Mutant

• BKE04800 ([gene|47AE982C5E71F31B5AF04C4F50593C2629F8EDAF|int]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTATTTCTCCTTTAAC,  downstream forward: _UP4_TAGGTAAGTGGGCAGTTTGT
• BKK04800 ([gene|47AE982C5E71F31B5AF04C4F50593C2629F8EDAF|int]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTATTTCTCCTTTAAC,  downstream forward: _UP4_TAGGTAAGTGGGCAGTTTGT

References

• 18005101,23667562