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  • Description: translation initiation factor IF-3

Gene name infC
Essential yes PubMed
Product initiation factor IF-3
Function translation
Gene expression levels in SubtiExpress: infC
MW, pI 19 kDa, 10.342
Gene length, protein length 519 bp, 173 aa
Immediate neighbours rpmI, yscB
Sequences Protein DNA DNA_with_flanks
Genetic context
InfC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
InfC expression.png


Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

RplT regulon, stringent response

The gene

Basic information

  • Locus tag: BSU28870

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: IF-3 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1TIF (N-terminal domain, Geobacillus stearothermophilus), 1TIG (C-terminal domain, Geobacillus stearothermophilus)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:
    • expression of IF3 is uncoupled from that of L35 and L20 by RNase Y-dependent processing PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 3318 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 9964 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks


Margaret M Elvekrog, Ruben L Gonzalez
Conformational selection of translation initiation factor 3 signals proper substrate selection.
Nat. Struct. Mol. Biol.: 2013, 20(5);628-33
[PubMed:23584454] [] [DOI] (I p)

Patrice Bruscella, Karen Shahbabian, Soumaya Laalami, Harald Putzer
RNase Y is responsible for uncoupling the expression of translation factor IF3 from that of the ribosomal proteins L35 and L20 in Bacillus subtilis.
Mol. Microbiol.: 2011, 81(6);1526-41
[PubMed:21843271] [] [DOI] (I p)

Ludek Sojka, Tomás Kouba, Ivan Barvík, Hana Sanderová, Zdenka Maderová, Jirí Jonák, Libor Krásny
Rapid changes in gene expression: DNA determinants of promoter regulation by the concentration of the transcription initiating NTP in Bacillus subtilis.
Nucleic Acids Res.: 2011, 39(11);4598-611
[PubMed:21303765] [] [DOI] (I p)

Nasslie Choonee, Sergine Even, Lena Zig, Harald Putzer
Ribosomal protein L20 controls expression of the Bacillus subtilis infC operon via a transcription attenuation mechanism.
Nucleic Acids Res.: 2007, 35(5);1578-88
[PubMed:17289755] [] [DOI] (I p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J. Bacteriol.: 2002, 184(9);2500-20
[PubMed:11948165] [] (P p)

V Biou, F Shu, V Ramakrishnan
X-ray crystallography shows that translational initiation factor IF3 consists of two compact alpha/beta domains linked by an alpha-helix.
EMBO J.: 1995, 14(16);4056-64
[PubMed:7664745] [] (P p)

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