ilvH

168

acetolactate synthase (small subunit)

locus: BSU_28300

Molecular weight: 19.31 kDa

pI: 10.3

Protein length: 172 aa

Gene length: 519 bp

function: biosynthesis of branched-chain amino acids

product: acetolactate synthase (small subunit)

essential: no

ec: 2.2.1.6

synonyms: ilvH, ilvN

List of homologs in different organisms, belongs to COG0440 (Galperin et al., 2021)

Gene

Coordinates: 2,894,733  2,895,251

Advertisement

The protein

Catalyzed reaction/ biological activity

• H⁺ + 2 pyruvate --> (2S)-2-acetolactate + CO₂ (according to UniProt)

Protein family

• acetolactate synthase small subunit family (single member, according to UniProt)

[wiki|Domains]

• [wiki|ACT domain] (aa 4-79) (according to UniProt)

Structure

• [PDB|2F1F] (regulatory subunit from ''Escherichia coli'', 37% identity, 62% similarity) [Pubmed|16458324]
• [AF|P37252]

Effectors of protein activity

• subject to feedback inhibition by valine [pubmed|29981237]

Biological materials

Mutant

• BKE28300 ([gene|49E2D2EE480C2FFDED0C35E3AF0DBED5AD819B5F|ilvH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAGTCAATGTGATAATTC,  downstream forward: _UP4_TAAAACATAACAAGGGAGAG
• BKK28300 ([gene|49E2D2EE480C2FFDED0C35E3AF0DBED5AD819B5F|ilvH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAGTCAATGTGATAATTC,  downstream forward: _UP4_TAAAACATAACAAGGGAGAG

References

• 15060025,12193635,19258532,8289305,18641142,15547269,12618455,12107147,20935095,25157083,24163341,25755103,26220295,29981237