ilvB

168

acetolactate synthase (large subunit)

locus: BSU_28310

Molecular weight: 62.42 kDa

pI: 5.18

Protein length: 574 aa

Gene length: 1725 bp

function: biosynthesis of branched-chain amino acids

product: acetolactate synthase (large subunit)

essential: no

ec: 2.2.1.6

synonyms: ilvB

List of homologs in different organisms, belongs to COG0028 (Galperin et al., 2021)

Gene

Coordinates: 2,895,248 → 2,896,972

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The protein

Catalyzed reaction/ biological activity

• H⁺ + 2 pyruvate --> (2S)-2-acetolactate + CO₂ (according to UniProt)

Protein family

• [wiki|TPP enzyme family] (according to UniProt)

[wiki|Cofactors]

• FAD (according to UniProt)
• TPP (according to UniProt)

Structure

• [PDB|4RJJ] [Pubmed|25393087]
• [PDB|1N53] (T box RNA)
• [AF|P37251]

Modification

• phosphorylated on Arg-297 [Pubmed|22517742]

Paralogous protein(s)

• [protein|2883FC60CD69948D5C43BDDFEFBE7A1CA7C743C7|ydaP], [protein|AAA45830B9C5428CADFA5551D33B513E9B1B7C8E|alsS]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

Expression and Regulation

Operons

• genes: [gene|E6EDDB6D1EDA85D73A0B78A656511D38346A625B|ilvB]-[gene|49E2D2EE480C2FFDED0C35E3AF0DBED5AD819B5F|ilvH]-[gene|EC1CB2FD563EFDA391D13EB8ADFDC8C9734E9D57|ilvC]-[gene|1502AED337F59DFFCEAFF68FE111C5AA6155701B|leuA]-[gene|105E3452D7F142A7D7616E35AF3FB753C9B63E38|leuB]-[gene|AC459429A9C50463FD947C1CF9EA919B6FE3B335|leuC]-[gene|0EB4C6EE398B14B9284FFAEDBC26C866CE2070D7|leuD]

  description: [Pubmed|1577690]

  regulation

  • expression is stimulated in the presence of glucose ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [PubMed|12193635]

  regulatory mechanism

  • T-box: RNA switch, via tRNA controlled [wiki|RNA switch], repression by BCAA, in [regulon|other_regulator:T-box|T-box]
  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: repression, [Pubmed|15547269], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [pubmed|12618455] [pubmed|18083814], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: activation, [Pubmed|12193635], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1577690], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

  additional information

  • An [wiki|ncRNA|antisense RNA] is predicted for [gene|1502AED337F59DFFCEAFF68FE111C5AA6155701B|leuA] [PubMed|20525796]
  • the [wiki|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

Biological materials

Mutant

• GP3927 (Δ[gene|E6EDDB6D1EDA85D73A0B78A656511D38346A625B|ilvB]::aphA3 trpC2), available in [wiki|Jörg Stülke]'s lab
• GP324, ''ilvB'' under control of pXyl (cat), available in [wiki|Jörg Stülke]'s lab
• BKE28310 (Δ[gene|E6EDDB6D1EDA85D73A0B78A656511D38346A625B|ilvB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28310 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTAGTTCCTCCTTTTG,  downstream forward: _UP4_CATGAAATGGTGGGGGTGAA
• BKK28310 (Δ[gene|E6EDDB6D1EDA85D73A0B78A656511D38346A625B|ilvB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28310 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTAGTTCCTCCTTTTG,  downstream forward: _UP4_CATGAAATGGTGGGGGTGAA

Expression vectors

• pGP2886: expression of Strep-''ilvB'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab

lacZ fusion

• pGP520 and pGP524 (both in [wiki|pAC5]), pGP523 (in [wiki|pAC6]), all are available in [wiki|Jörg Stülke]'s lab

References

Reviews

• 35988322

Original Publications

• 21303765,15060025,19258532,8289305,17488331,18083814,18641142,24163341,15547269,12618455,12107147,1577690,12193635,15916605,15916606,20935095,22517742,22900538,25157083,25755103,26220295,28516784,29794222,25393087