hxlB

168

6-phospho-3-hexuloisomerase

locus: BSU_03450

Molecular weight: 19.82 kDa

pI: 5.79

Protein length: 185 aa

Gene length: 558 bp

function: ribulose monophosphate pathway for formaldehyde fixation

product: 6-phospho-3-hexuloisomerase

essential: no

ec: 5.3.1.27

synonyms: hxlB, yckF

List of homologs in different organisms, belongs to COG0794 (Galperin et al., 2021)

Gene

Coordinates: 374,603 → 375,160

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The protein

Catalyzed reaction/ biological activity

• D-arabino-hex-3-ulose 6-phosphate --> β-D-fructose 6-phosphate (according to UniProt)

Protein family

• SIS family (single member, according to UniProt)

[wiki|Domains]

• [wiki|SIS domain] (aa 29-172) (according to UniProt)

Structure

• [PDB|1M3S] [pubmed|15363790]
• [AF|P42404]

Expression and Regulation

Operons

• genes: [gene|BF480721B89661BFFE6632BB10C5F4DC29951784|hxlA]-[gene|FB0CEA291CA4EC5FB8410050E61B89EDBA1771F9|hxlB]

  description: [Pubmed|10572115]

  regulation

  • induction by formaldehyde ([protein|search|HxlR]) [Pubmed|10572115]

  regulatory mechanism

  • [protein|D9A187961CFB9496A6712E9E48AAD384357A3E1C|hxlR]: activation, [Pubmed|10572115], in [regulon|protein:D9A187961CFB9496A6712E9E48AAD384357A3E1C|hxlR regulon]

Biological materials

Mutant

• MGNA-C056 (yckF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2054 NBRP B. subtilis, Japan]
• BKE03450 (Δ[gene|FB0CEA291CA4EC5FB8410050E61B89EDBA1771F9|hxlB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTATTCAGTCGTTTTCATCG,  downstream forward: _UP4_TAGCATCACACAACCGGCCT
• BKK03450 (Δ[gene|FB0CEA291CA4EC5FB8410050E61B89EDBA1771F9|hxlB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTATTCAGTCGTTTTCATCG,  downstream forward: _UP4_TAGCATCACACAACCGGCCT

References

• 10572115,11468398,16428816,19170879,15363790