gsaB

168

glutamate-1-semialdehyde aminotransferase, required for [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] modification

locus: BSU_08710

Molecular weight: 46.04 kDa

pI: 5.64

Protein length: 429 aa

Gene length: 1290 bp

function: biosynthesis of heme, modification of [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]

product: glutamate-1-semialdehyde aminotransferase

essential: no

ec: 5.4.3.8

synonyms: gsaB

List of homologs in different organisms, belongs to COG0001 (Galperin et al., 2021)

Gene

Coordinates: 942,449  943,738

Phenotypes of a mutant

• inactivation suppresses the [category|SW.4.1.4|Swarming] defect of a [gene|248F0805272FED9B38ECBB31E2872BC9EC163CE0|ymfI] mutant [pubmed|29615499]
• defective in [category|SW.4.1.4|Swarming] motility [pubmed|35638827]

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The protein

Catalyzed reaction/ biological activity

• (S)-4-amino-5-oxopentanoate --> 5-aminolevulinate (according to UniProt)

Protein family

• [wiki|Class-III pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

[wiki|Cofactors]

• pyridoxal phosphate (attached to Lys-267) (according to UniProt)

Structure

• [PDB|3BS8] ([protein|A54B2C86638D474F5F892003B42C535B5B39A42E|hemL]) [Pubmed|20946885]
• [AF|P71084]

Modification

• pyridoxal phosphate attached to Lys-267 (according to UniProt)

Paralogous protein(s)

• [protein|A54B2C86638D474F5F892003B42C535B5B39A42E|hemL]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|8BB8EECA8ECFA1CC6D46E81250905DBC6D2C503E|gsaB]

  description: [pubmed|22383849]

Biological materials

Mutant

• BKE08710 ([gene|8BB8EECA8ECFA1CC6D46E81250905DBC6D2C503E|gsaB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08710 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGAATAAAAACCTCCTAT,  downstream forward: _UP4_TAGAATCAAATACCTTGAAC
• BKK08710 ([gene|8BB8EECA8ECFA1CC6D46E81250905DBC6D2C503E|gsaB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08710 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGAATAAAAACCTCCTAT,  downstream forward: _UP4_TAGAATCAAATACCTTGAAC

References

• 20946885,29615499,35638827