gpsA

gpsA
168

glycerol-3-phosphate dehydrogenase (NAD)

locus
BSU_22830
Molecular weight
37.28 kDa
pI
6.23
Protein length
Gene length
function
biosynthesis of phospholipids
product
glycerol-3-phosphate dehydrogenase (NAD)
essential
no
ec
1.1.1.94
synonyms
gpsA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0240 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,389,151  2,390,188
Phenotypes of a mutant
essential [Pubmed|12682299], non-essential according to [Pubmed|28189581]
poor growth [pubmed|28189581]
poorly transformable [pubmed|28189581]
defective in biofilm formation [pubmed|31420537]
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The protein
Catalyzed reaction/ biological activity
NAD+ + sn-glycerol 3-phosphate --> dihydroxyacetone phosphate + H+ + NADH (according to UniProt)
NADP+ + sn-glycerol 3-phosphate --> dihydroxyacetone phosphate + H+ + NADPH (according to UniProt)
Protein family
NAD-dependent glycerol-3-phosphate dehydrogenase family (single member, according to UniProt)
Structure
[PDB|3K96] (from ''Coxiella burnetii RSA 493'', 37% identity, 59% similarity)
[AF|P46919]
[wiki|Localization]
cytoplasm [Pubmed|15743965]
Expression and Regulation
Operons
genes
[gene|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|engA]-[gene|8440C1DBE485BB9EF2904FAA5D709A574C3819A4|gpsA]
description
[pubmed|22383849]
Open in new tab

[gene|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|engA]→[gene|8440C1DBE485BB9EF2904FAA5D709A574C3819A4|gpsA]

2024-03-23 16:38:41

Jstuelk

79

e742857757b54cfd3dbd31d4c2a8732ae1d6f10f

447F965D334A595E929D73835DC559433A0B536E

genes
[gene|5DA8B27AC6A0E0B4115512BD948C31964D387A8E|yphA]-[gene|448375DF74EB0482A6CD1ACFE04D117C1319E2B2|seaA]-[gene|910109496E4A41EC892996DEEB81971FCBD9CB05|ypzH]-[gene|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|engA]-[gene|8440C1DBE485BB9EF2904FAA5D709A574C3819A4|gpsA]
description
[pubmed|22383849]
Open in new tab

[gene|5DA8B27AC6A0E0B4115512BD948C31964D387A8E|yphA]→[gene|8440C1DBE485BB9EF2904FAA5D709A574C3819A4|gpsA]

2024-03-23 16:39:09

Jstuelk

82

59d7f332f8e66c2f3ab828b44d7b5670bff82244

CC6F80D7EB8E18FBBD197DB0376D1D6AFBFC5A02

additional information
highly expressed at high iron concentrations [pubmed|31420537]
Biological materials
Mutant
BKE22830 ([gene|8440C1DBE485BB9EF2904FAA5D709A574C3819A4|gpsA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22830 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTCATGTTTGATTCACACC,  downstream forward: _UP4_TAAAAGTGTCAATCAAATGG
BKK22830 ([gene|8440C1DBE485BB9EF2904FAA5D709A574C3819A4|gpsA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22830 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTCATGTTTGATTCACACC,  downstream forward: _UP4_TAAAAGTGTCAATCAAATGG
References
7592341,15743965,28189581,31420537

8440C1DBE485BB9EF2904FAA5D709A574C3819A4

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Time of last update: 2024-03-28 04:03:15

Author of last update: Jstuelk