glgP

168

glycogen phosphorylase

locus: BSU_30940

Molecular weight: 91.56 kDa

pI: 6.28

Protein length: 798 aa

Gene length: 2394 bp

function: glycogen biosynthesis

product: glycogen phosphorylase

essential: no

ec: 2.4.1.1

synonyms: glgP

List of homologs in different organisms, belongs to COG0058 (Galperin et al., 2021)

Gene

Coordinates: 3,163,735 → 3,166,131

Advertisement

The protein

Catalyzed reaction/ biological activity

• [(1→4)-α-D-glucosyl]_(n) + phosphate --> [(1→4)-α-D-glucosyl]_(n-1) + α-D-glucose 1-phosphate (according to UniProt)

Protein family

• glycogen phosphorylase family (single member, according to UniProt)

[wiki|Cofactors]

• PLP (according to UniProt)

Structure

• [PDB|1PYG] (from Oryctolagus cuniculus, 45% identity) [pubmed|1962195]
• [AF|P39123]

Modification

• phosphorylation on (Thr-291 OR Ser-294) [Pubmed|17218307]

Expression and Regulation

Operons

• genes: [gene|52C8DE3C78B680C8883D1CD559CBC2D9ABFCC400|glgB]-[gene|B665735B76462724A3C7A1E89D3E613C18F21399|glgC]-[gene|63A4FD35A2E72C665BFA3F2632F06C7A0C954087|glgD]-[gene|EBEA85AD543B79C321CB5F28D1B7E1248640DC1D|glgA]-[gene|3AB4513126EFF54996A54CB7DEB6062C1A06AAC3|glgP]

  description: [Pubmed|8145641]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|8145641]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|8145641], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]

Biological materials

Mutant

• BKE30940 (Δ[gene|3AB4513126EFF54996A54CB7DEB6062C1A06AAC3|glgP]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30940 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TAAAAATAAGTCTGCAAAGC,  downstream forward: _UP4_TGAAAAAGCCGCTGTATAAG
• BKK30940 (Δ[gene|3AB4513126EFF54996A54CB7DEB6062C1A06AAC3|glgP]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30940 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TAAAAATAAGTCTGCAAAGC,  downstream forward: _UP4_TGAAAAAGCCGCTGTATAAG

References

• 17218307,8145641,1962195