glgC

168

glucose-1-phosphate adenylyltransferase

locus: BSU_30970

Molecular weight: 42.44 kDa

pI: 4.86

Protein length: 380 aa

Gene length: 1143 bp

function: glycogen biosynthesis

product: glucose-1-phosphate adenylyltransferase

essential: no

ec: 2.7.7.27

synonyms: glgC

List of homologs in different organisms, belongs to COG0448 (Galperin et al., 2021)

Gene

Coordinates: 3,168,624 → 3,169,766

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The protein

Catalyzed reaction/ biological activity

• α-D-glucose 1-phosphate + ATP + H⁺ --> ADP-α-D-glucose + diphosphate (according to UniProt)

Protein family

• bacterial/plant glucose-1-phosphate adenylyltransferase family (with [protein|63A4FD35A2E72C665BFA3F2632F06C7A0C954087|glgD], according to UniProt)

Structure

• [PDB|5L6S] (E. coli ADP-glucose pyrophosphorylase, 41% identity) [pubmed|27545622]
• [AF|P39122]

Paralogous protein(s)

• [protein|63A4FD35A2E72C665BFA3F2632F06C7A0C954087|glgD]

Expression and Regulation

Operons

• genes: [gene|52C8DE3C78B680C8883D1CD559CBC2D9ABFCC400|glgB]-[gene|B665735B76462724A3C7A1E89D3E613C18F21399|glgC]-[gene|63A4FD35A2E72C665BFA3F2632F06C7A0C954087|glgD]-[gene|EBEA85AD543B79C321CB5F28D1B7E1248640DC1D|glgA]-[gene|3AB4513126EFF54996A54CB7DEB6062C1A06AAC3|glgP]

  description: [Pubmed|8145641]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|8145641]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|8145641], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]

Biological materials

Mutant

• BKE30970 (Δ[gene|B665735B76462724A3C7A1E89D3E613C18F21399|glgC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GAGCATGGCTACACATTGTT,  downstream forward: _UP4_TAATTACTGAAGAGGGGGCA
• BKK30970 (Δ[gene|B665735B76462724A3C7A1E89D3E613C18F21399|glgC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GAGCATGGCTACACATTGTT,  downstream forward: _UP4_TAATTACTGAAGAGGGGGCA

References

• 8145641,27545622