gerBA

168

nutrient receptor, germination response to the combination of glucose, fructose, and KCl

locus: BSU_35800

Molecular weight: 53.62 kDa

pI: 7.3

Protein length: 483 aa

Gene length: 1452 bp

function: germination response to the combination of glucose, fructose, and KCl

product: part of the putative nutrient-gated [protein|3368743E6E03792DB83A38A19989123304DF7560|gerBA]-[protein|D70EC7C02BC79BED724489ABCB6356447F215B87|gerBB]-[protein|93D4D6864686E6E559E6CEFA69BAC77EEFAA1BE3|gerBC] ion channel

essential: no

synonyms: gerBA

List of homologs in different organisms, belongs to COG5901 (Galperin et al., 2021)

Gene

Coordinates: 3,688,812  3,690,263

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The protein

Catalyzed reaction/ biological activity

• the [protein|3368743E6E03792DB83A38A19989123304DF7560|gerBA]-[protein|D70EC7C02BC79BED724489ABCB6356447F215B87|gerBB]-[protein|93D4D6864686E6E559E6CEFA69BAC77EEFAA1BE3|gerBC] ion channel likely releases ions from the spore core in response to asparagine, glucose, fructose and K⁺ to trigger [category|SW.4.2.4|Germination], this requires the gerK locus [pubmed|37104613]

Protein family

• [wiki|GerABKA family] (according to UniProt)

Structure

• [PDB|6O59] (from B. megaterium, corresponds to aa 7 ... 264, 36.5% identity) [pubmed|31113879]
• [AF|P39569]

Paralogous protein(s)

• [protein|7CE0CAF27B1C0F507E783B8B80622270B00634FD|yfkQ], [protein|AE28299B0F274B189DD90554A5A4F7B9A9479B60|yndD], [protein|EDB2F4C132211F01654F2A2ED3D220EC15CE6F22|gerKA], [protein|EEDE6A22E91992ABCA677CAB7917B7168C5CB868|gerAA]

[wiki|Localization]

• spore inner membrane [Pubmed|21696470]

Expression and Regulation

Operons

• genes: [gene|3368743E6E03792DB83A38A19989123304DF7560|gerBA]-[gene|D70EC7C02BC79BED724489ABCB6356447F215B87|gerBB]-[gene|93D4D6864686E6E559E6CEFA69BAC77EEFAA1BE3|gerBC]

  description: [Pubmed|8012571]

  regulation

  • expressed late during sporulation in the forespore ([protein|search|SigG]) [Pubmed|15699190,8012571]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|15699190,8012571], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]

  additional information

  • 700 molecules are present per spore [PubMed|23749970]

Biological materials

Mutant

• BKE35800 ([gene|3368743E6E03792DB83A38A19989123304DF7560|gerBA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGTTTCTCTCCTTCTTA,  downstream forward: _UP4_AACATCAGACAAAGGTGATG
• BKK35800 ([gene|3368743E6E03792DB83A38A19989123304DF7560|gerBA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGTTTCTCTCCTTCTTA,  downstream forward: _UP4_AACATCAGACAAAGGTGATG

References

• 36815589,10348844,12670969,15774895,7812448,11395462,16352818,8012571,21696470,23396907,24752279,16740944,26731423,31113879,37104613