FosB

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  • Description: bacillithiol-S-transferase; confers resistence against antimicrobial compounds from B. amyloliquefaciens

Gene name fosB
Synonyms yndN
Essential no
Product fosfomycin resistance protein
Function confers resistence against antimicrobial compounds
from B. amyloliquefaciens
Gene expression levels in SubtiExpress: fosB
MW, pI 17 kDa, 6.137
Gene length, protein length 432 bp, 144 aa
Immediate neighbours yndM, lexA
Sequences Protein DNA DNA_with_flanks
Genetic context
YndN context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FosB expression.png

























Categories containing this gene/protein

cell envelope stress proteins (controlled by SigM, V, W, X, Y), resistance against toxins/ antibiotics

This gene is a member of the following regulons

SigW regulon

The gene

Basic information

  • Locus tag: BSU17840

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: FosB subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Alexandra A Roberts, Sunil V Sharma, Andrew W Strankman, Shayla R Duran, Mamta Rawat, Chris J Hamilton
Mechanistic studies of FosB: a divalent-metal-dependent bacillithiol-S-transferase that mediates fosfomycin resistance in Staphylococcus aureus.
Biochem. J.: 2013, 451(1);69-79
[PubMed:23256780] [WorldCat.org] [DOI] (I p)

Alexander P Lamers, Mary E Keithly, Kwangho Kim, Paul D Cook, Donald F Stec, Kelly M Hines, Gary A Sulikowski, Richard N Armstrong
Synthesis of bacillithiol and the catalytic selectivity of FosB-type fosfomycin resistance proteins.
Org. Lett.: 2012, 14(20);5207-9
[PubMed:23030527] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res.: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Bronwyn G Butcher, John D Helmann
Identification of Bacillus subtilis sigma-dependent genes that provide intrinsic resistance to antimicrobial compounds produced by Bacilli.
Mol. Microbiol.: 2006, 60(3);765-82
[PubMed:16629676] [WorldCat.org] [DOI] (P p)

M Cao, B A Bernat, Z Wang, R N Armstrong, J D Helmann
FosB, a cysteine-dependent fosfomycin resistance protein under the control of sigma(W), an extracytoplasmic-function sigma factor in Bacillus subtilis.
J. Bacteriol.: 2001, 183(7);2380-3
[PubMed:11244082] [WorldCat.org] [DOI] (P p)

23256780