fhuG

168

hydroxamate siderophore [wiki|ABC transporter ](ferrichrome und ferrioxamine) (permease)

locus: BSU_33300

Molecular weight: 36.00 kDa

pI: 10.2

Protein length: 336 aa

Gene length: 1011 bp

function: siderophore uptake

product: hydroxamate siderophore [wiki|ABC transporter ](ferrichrome und ferrioxamine) (permease)

essential: no

synonyms: fhuG

List of homologs in different organisms, belongs to COG0609 (Galperin et al., 2021)

Gene

Coordinates: 3,416,212 → 3,417,222

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The protein

Protein family

• [wiki|Binding-protein-dependent transport system permease family] (according to UniProt)
• [wiki|FecCD subfamily] (according to UniProt)

Structure

• [PDB|4R9U], the ''E. coli'' BtuC-BtuD complex, BtuC shares 33% identity, 67% similarity with FhuG, [Pubmed|25402482]
• [AF|P49937]

Paralogous protein(s)

• [protein|F38F6D4841B3044EA0496B3A1C1D484018BDFEA4|feuC], [protein|34A7E22B4EF118EF5A76F8CC2DABBB023BC9A2E9|yfhA], [protein|800EAB1D8617589CCAC68FB874EFCDE4C77962E1|fecE]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|3E1C925C31C00803982C8EB1C6B025C22DB4A828|fhuB]-[gene|EC98685EE0E3CE6CE8DC33409481AE96315279AC|fhuG]-[gene|E70BE74D5AB4B06CDFF902E58A0B9EBF477E21EC|fhuC]

  description

  • [pubmed|22383849]

  regulation

  • induced by iron starvation (second wave to allow iron scavenging from the environment) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]) [pubmed|29133393,12354229]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]

Biological materials

Mutant

• BKE33300 (Δ[gene|EC98685EE0E3CE6CE8DC33409481AE96315279AC|fhuG]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGGTTTGTTTGTTTTTGTTT,  downstream forward: _UP4_GCGAATTAGAGGAGGGGGCC
• BKK33300 (Δ[gene|EC98685EE0E3CE6CE8DC33409481AE96315279AC|fhuG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGGTTTGTTTGTTTTTGTTT,  downstream forward: _UP4_GCGAATTAGAGGAGGGGGCC

References

• 10092453,16672620,25402482,12354229,29133393