epsI

168

glycosyltransferase, synthesis of extracellular poly-N-acetylglucosamine

locus: BSU_34290

Molecular weight: 41.35 kDa

pI: 8.04

Protein length: 358 aa

Gene length: 1077 bp

function: synthesis of extracellular poly-N-acetylglucosamine

product: glycosyltransferase

essential: no

synonyms: epsI, yveS

List of homologs in different organisms, belongs to COG5039 (Galperin et al., 2021)

Gene

Coordinates: 3,520,030  3,521,106

The EAR [wiki|RNA switch]

• 20374491,20230605

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The protein

Protein family

• polysaccharide pyruvyl transferase family (with [protein|52A47B49B1FB955EE7E3C316B57A2B4134F78480|epsO] and [protein|9A223AE85131511B7EA3A71EA57D87E8B167FA67|yxaB], according to UniProt)

Structure

• [AF|P71058]

Paralogous protein(s)

• [protein|9A223AE85131511B7EA3A71EA57D87E8B167FA67|yxaB]

Biological materials

Mutant

• MGNA-B612 (yveS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1611 NBRP B. subtilis, Japan]
• BKE34290 ([gene|6EBD8F7065B6C1996067F3120DD9CFEA91D3A444|epsI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34290 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCCTCACCCTCTGTTT,  downstream forward: _UP4_CAATGATCCCGCTCGTCAGC
• BKK34290 ([gene|6EBD8F7065B6C1996067F3120DD9CFEA91D3A444|epsI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34290 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCCTCACCCTCTGTTT,  downstream forward: _UP4_CAATGATCCCGCTCGTCAGC

labs

• [wiki|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]

References

Reviews

• 20735481