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  • Description: lipoprotein, elemental iron uptake system (binding protein), high affinity uptake of ferric iron (Fe(III))

Gene name ywbM
Synonyms ipa-28d
Essential no
Product elemental iron uptake system (binding protein)
Function elemental iron uptake
Gene expression levels in SubtiExpress: efeO
Interactions involving this protein in SubtInteract: EfeO
Metabolic function and regulation of this protein in SubtiPathways:
MW, pI 42 kDa, 4.712
Gene length, protein length 1155 bp, 385 aa
Immediate neighbours efeB, efeU
Sequences Protein DNA DNA_with_flanks
Genetic context
YwbM context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YwbM expression.png

Categories containing this gene/protein

transporters/ other, acquisition of iron, iron metabolism, membrane proteins

This gene is a member of the following regulons

Fur regulon

The gene

Basic information

  • Locus tag: BSU38270

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • binds ferric iron (Fe(III)) with strong selectivity PubMed
  • Protein family: UPF0409 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 1047 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 2113 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks


Marcus Miethke, Carmine G Monteferrante, Mohamed A Marahiel, Jan Maarten van Dijl
The Bacillus subtilis EfeUOB transporter is essential for high-affinity acquisition of ferrous and ferric iron.
Biochim. Biophys. Acta: 2013, 1833(10);2267-78
[PubMed:23764491] [WorldCat.org] [DOI] (P p)

Carmine G Monteferrante, Calum MacKichan, Elodie Marchadier, Maria-Victoria Prejean, Rut Carballido-López, Jan Maarten van Dijl
Mapping the twin-arginine protein translocation network of Bacillus subtilis.
Proteomics: 2013, 13(5);800-11
[PubMed:23180473] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Juliane Ollinger, Kyung-Bok Song, Haike Antelmann, Michael Hecker, John D Helmann
Role of the Fur regulon in iron transport in Bacillus subtilis.
J. Bacteriol.: 2006, 188(10);3664-73
[PubMed:16672620] [WorldCat.org] [DOI] (P p)

Noel Baichoo, Tao Wang, Rick Ye, John D Helmann
Global analysis of the Bacillus subtilis Fur regulon and the iron starvation stimulon.
Mol. Microbiol.: 2002, 45(6);1613-29
[PubMed:12354229] [WorldCat.org] [DOI] (P p)

E Presecan, I Moszer, L Boursier, H C Cruz Ramos, V de la Fuente, M F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading): 1997, 143 ( Pt 10);3313-28
[PubMed:9353933] [WorldCat.org] [DOI] (P p)