cypX

cypX
168

cytochrome P450, cyclo-l-leucyl-l-leucyl dipeptide oxidase

locus
BSU_35060
Molecular weight
45.31 kDa
pI
5.31
Protein length
Gene length
function
biosynthesis of the extracellular iron chelator pulcherrimin
product
cytochrome P450, cyclo-l-leucyl-l-leucyl dipeptide oxidase
essential
no
synonyms
cypX

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2124 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,602,588 → 3,603,805
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The protein
Catalyzed reaction/ biological activity
implicated in the three-step oxidative transformation of the diketopiperazine cyclo-l-leucyl-l-leucyl into pulcherriminic acid [Pubmed|20690619]
cyclo(L-leucyl-L-leucyl) + 4 H+ + 3 O2 + 6 reduced [2Fe-2S]-[ferredoxin] --> 4 H2O + 6 oxidized [2Fe-2S]-[ferredoxin] + pulcherriminic acid (according to UniProt)
Protein family
[wiki|cytochrome P450] family (according to UniProt)
Structure
[PDB|3NC3] [Pubmed|20690619]
[AF|O34926]
Expression and Regulation
Operons
genes
[gene|BCE1D8CDDF07396583FF50C4C192785545244BE2|yvmC]-[gene|D63EDBB6AA33708E8352F6208A9BA18CAEE0DC53|cypX]
description
[http://www.liebertonline.com/doi/pdf/10.1089/ind.2006.2.66 Reference]
expression
expression is heterogeneous [pubmed|35171018]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|38260623,20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
[protein|0D555F2AB7DC863E6FF388888308E980514DB719|pchR]: repression, [pubmed|38260623], in [regulon|protein:0D555F2AB7DC863E6FF388888308E980514DB719|pchR regulon]
[protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC]: repression, [pubmed|38260623], in [regulon|protein:F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC regulon]
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[gene|BCE1D8CDDF07396583FF50C4C192785545244BE2|yvmC]→[gene|D63EDBB6AA33708E8352F6208A9BA18CAEE0DC53|cypX]

2024-03-22 03:26:47

Jstuelk

42

0335F16608554630BB979B2EC3E5321773D5D3C3

3B02729FC2C4AD75A2BFE99E2ED90819DABC19B4

Biological materials
Mutant
MGNA-A333 (cypX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/333 NBRP B. subtilis, Japan]
BKE35060 (Δ[gene|D63EDBB6AA33708E8352F6208A9BA18CAEE0DC53|cypX]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35060 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTGGCTCATGTTTTACTCCC,  downstream forward: _UP4_TAATAGAATTCCAAAGGTCT
BKK35060 (Δ[gene|D63EDBB6AA33708E8352F6208A9BA18CAEE0DC53|cypX]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35060 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTGGCTCATGTTTTACTCCC,  downstream forward: _UP4_TAATAGAATTCCAAAGGTCT
References
20690619,20817675,4204912,27542896,35171018

D63EDBB6AA33708E8352F6208A9BA18CAEE0DC53

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Time of last update: 2024-03-28 19:50:32

Author of last update: Jstuelk