cwlQ

cwlQ
168

putative cell wall hydrolase (N-acetylglucosamidase), required for swarming motility

locus
BSU_11570
Molecular weight
18.85 kDa
pI
7.01
Protein length
Gene length
function
cell wall turnover
product
lytic transglycosylase
essential
no
synonyms
cwlQ, yjbJ

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0741 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,235,163 → 1,235,708
Phenotypes of a mutant
severe defect in flagellar-dependent [category|SW.4.1.4|Swarming] motility [pubmed|33649146,35638827]
Visit Sartorius.com Visit Sartorius.com
Advertisement
The protein
Catalyzed reaction/ biological activity
bifunctional cell wall hydrolase with muramidase and soluble-lytic transglycosylase activities [Pubmed|20609359]
Protein family
transglycosylase Slt family (together with [protein|F43DC974DC71CB5F82DBD9B9D5ACEB49131772DB|cwlP]) (according to UniProt)
[wiki|Domains]
unkown N-terminal domain [pubmed|33649146]
C-terminal lytic transglycosidase domain (aa 63 - 244) [pubmed|33649146]
Structure
[PDB|5OHU] (from Pseudomonas aeruginosa, corresponds to aa 62 ... 177, 37% identity) [pubmed|29632171]
[AF|O31608]
[wiki|Localization]
secreted by the flagellar type III secretion system involving [protein|EB815705133E3318F655F6024B7D9BA587FD1DDA|fliF] [pubmed|33649146]
Expression and Regulation
Operons
genes
[gene|BD3C36B63C5D88FDBACFB18849F3DA863F38383F|cwlQ]
description
[Pubmed|15033535]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|15033535], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
Open in new tab

[gene|BD3C36B63C5D88FDBACFB18849F3DA863F38383F|cwlQ]

2024-03-14 05:59:38

ghost

50

060c03d2b9927ca3ba189d1bdd9200c81d731ae1

B18DD478E6CE4A342E98F86FF593B00945146C25

genes
[gene|38EABA6E1998FF194B6EEB6ACDC800615E998BD3|yjbK]-[gene|BD3C36B63C5D88FDBACFB18849F3DA863F38383F|cwlQ]
description
[Pubmed|22383849]
Open in new tab

[gene|38EABA6E1998FF194B6EEB6ACDC800615E998BD3|yjbK]→[gene|BD3C36B63C5D88FDBACFB18849F3DA863F38383F|cwlQ]

2024-03-12 05:35:46

Jstuelk

46

fc6bbba29265345014e99da944ae0e1800e79f98

F686CB4F40746272B66DB966F83FD64C002C491E

Biological materials
Mutant
MGNA-B156 (yjbJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1155 NBRP B. subtilis, Japan]
BKE11570 (Δ[gene|BD3C36B63C5D88FDBACFB18849F3DA863F38383F|cwlQ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11570 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCTGAGCGGGCTTGGCG,  downstream forward: _UP4_TAACCAGAGGTGCTCTTTCC
BKK11570 (Δ[gene|BD3C36B63C5D88FDBACFB18849F3DA863F38383F|cwlQ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11570 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCTGAGCGGGCTTGGCG,  downstream forward: _UP4_TAACCAGAGGTGCTCTTTCC
References
20609359,15033535,29632171,33649146,35638827

BD3C36B63C5D88FDBACFB18849F3DA863F38383F

Page visits: 3839

Time of last update: 2024-03-28 10:31:19

Author of last update: Jstuelk