cspR

cspR
168

tRNA (Um34/Cm34) methyltransferase

locus
BSU_08930
Molecular weight
18.44 kDa
pI
6.05
Protein length
Gene length
function
translation
product
tRNA (Um34/Cm34) methyltransferase
essential
no
ec
2.1.1.-
synonyms
cspR, ygaR, trmL

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0219 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
970,135  970,617
Phenotypes of a mutant
essential [Pubmed|12682299], non-essential according to [Pubmed|28189581]
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The protein
Catalyzed reaction/ biological activity
catalyzes the methyl transfer from S-adenosyl-L-methionine to tRNA(Leu)(CAA) and tRNA(Leu)(UAA) isoacceptors [Pubmed|23804755]
cytidine34 in tRNA + S-adenosyl-L-methionine --> 2'-O-methylcytidine34 in tRNA + H+ + S-adenosyl-L-homocysteine (according to UniProt)
5-carboxymethylaminomethyluridine34 in tRNALeu + S-adenosyl-L-methionine --> 5-carboxymethylaminomethyl-2'-O-methyluridine34 in tRNALeu + H+ + S-adenosyl-L-homocysteine (according to UniProt)
Protein family
[wiki|Methyltransferase superfamily] (according to UniProt)
class IV-like SAM-binding methyltransferase superfamily (with [protein|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|yacO] and [protein|8A2898B80D0FFA56C5AA86C6F09D886A62AABBDA|rlmP], according to UniProt)
Structure
[PDB|4JAK] (from ''E. coli'', 45% identity, 72% similarity) [Pubmed|23804755]
[AF|O31590]
Expression and Regulation
Operons
genes
[gene|506BD8F55C235CCCF5045073AEC93D8B1E4FB8DF|yhbB]-[gene|03BA7AEE17739689D632A35D9C3329EA0982C98A|cspR]
description
[Pubmed|12662922]
regulation
''[wiki|yhbB]-[wiki|cspR]'': expressed in the mother cell early during [wiki|sporulation] ([wiki|SigE]) [Pubmed|12662922]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|12662922], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Open in new tab

[gene|506BD8F55C235CCCF5045073AEC93D8B1E4FB8DF|yhbB]→[gene|03BA7AEE17739689D632A35D9C3329EA0982C98A|cspR]

2024-03-16 18:48:24

ghost

54

29b96ca9161089a03a179025bb3ceee0647ec726

5B631CC6ACD9F6DCA4410FD54B73BD6CAF63882A

genes
[gene|03BA7AEE17739689D632A35D9C3329EA0982C98A|cspR]
description
[Pubmed|22383849]
regulation
''[wiki|yhbB]-[wiki|cspR]'': expressed in the mother cell early during [wiki|sporulation] ([wiki|SigE]) [Pubmed|12662922]
Open in new tab

[gene|03BA7AEE17739689D632A35D9C3329EA0982C98A|cspR]

2024-03-18 04:38:40

ghost

61

0d8447cdc61b9de5b89e45a9fd00a5d7ee8731ec

9FF31973E2083617EC131EB97918554671B0A792

Biological materials
Mutant
BKE08930 ([gene|03BA7AEE17739689D632A35D9C3329EA0982C98A|cspR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08930 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACCTCTGTTTTTCTTATCC,  downstream forward: _UP4_TAGTGAAAAACCCGCTCATC
BKK08930 ([gene|03BA7AEE17739689D632A35D9C3329EA0982C98A|cspR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08930 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACCTCTGTTTTTCTTATCC,  downstream forward: _UP4_TAGTGAAAAACCCGCTCATC
References
23804755,12662922,22383849,28189581

03BA7AEE17739689D632A35D9C3329EA0982C98A

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Time of last update: 2024-03-29 03:51:04

Author of last update: Jstuelk