cotR

168

outer spore coat protein, lipolytic enzyme

locus: BSU_34530

Molecular weight: 35.20 kDa

pI: 10.2

Protein length: 320 aa

Gene length: 963 bp

function: hydrolysis of lysophospholipids

product: outer spore coat lipolytic enzyme

essential: no

synonyms: cotR, yvdO

List of homologs in different organisms, belongs to COG3621 (Galperin et al., 2021)

Gene

Coordinates: 3,544,642  3,545,604

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The protein

Catalyzed reaction/ biological activity

• release of myristic acid from 1-myristoly-2-lyso-sn-glycero-3-phosphocholine [Pubmed|20378985]

[wiki|Domains]

• PNPLA domain (aa 7-182) (according to UniProt)

Structure

• [AF|O06996]

[wiki|Localization]

• outer spore coat [pubmed|28870294]

Biological materials

Mutant

• MGNA-B621 (yvdO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1620 NBRP B. subtilis, Japan]
• BKE34530 ([gene|6B9B62D9687331CDC2677C78A624FA609C8F8CFC|cotR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34530 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAAGCTGATGTCTCCTTTC,  downstream forward: _UP4_TAATTATTTATTTTAAATCA
• BKK34530 ([gene|6B9B62D9687331CDC2677C78A624FA609C8F8CFC|cotR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34530 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAAGCTGATGTCTCCTTTC,  downstream forward: _UP4_TAATTATTTATTTTAAATCA

References

• 11737650,15699190,20378985,28870294,33596081