cotM

168

spore coat protein (outer)

locus: BSU_17970

Molecular weight: 15.08 kDa

pI: 3.94

Protein length: 130 aa

Gene length: 393 bp

function: resistance of the spore

product: spore coat protein (outer)

essential: no

synonyms: cotM, yneL

List of homologs in different organisms, belongs to COG0071 (Galperin et al., 2021)

Gene

Coordinates: 1,925,655 → 1,926,047

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The protein

Structure

• [AF|Q45058]

[wiki|Localization]

• outer spore coat, localization depends on [protein|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE] [Pubmed|22171814]

Expression and Regulation

Operons

• genes: [gene|DB8DC5B305DB5F301B047A2CA2089EC6485E7109|cotM]

  description: [Pubmed|9068633]

  regulation

  • expressed during sporulation in the mother cell ([protein|search|SigE], [protein|search|SigK], [protein|search|GerE]) [Pubmed|15383836,9068633]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]: repression, [Pubmed|9068633], in [regulon|protein:19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE regulon]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15383836], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|9068633], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]

  additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|yneK]' and '[protein|search|cotM]' [PubMed|20525796]

Biological materials

Mutant

• BKE17970 (Δ[gene|DB8DC5B305DB5F301B047A2CA2089EC6485E7109|cotM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCAACCTTGCTTTACA,  downstream forward: _UP4_TAAACATAAAAAGTCACTTT
• BKK17970 (Δ[gene|DB8DC5B305DB5F301B047A2CA2089EC6485E7109|cotM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCAACCTTGCTTTACA,  downstream forward: _UP4_TAAACATAAAAAGTCACTTT

References

Reviews

• 23202530

Original Publications

• 11150673,9068633,20525796,15383836,22171814,28870294