cotD

168

spore coat protein (inner)

locus: BSU_22200

Molecular weight: 8.70 kDa

pI: 7.37

Protein length: 75 aa

Gene length: 228 bp

function: resistance of the spore

product: spore coat protein (inner)

essential: no

ec: null

synonyms: cotD

List of homologs in different organisms, belongs to COG5879 (Galperin et al., 2021)

Gene

Coordinates: 2,332,784 → 2,333,011

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The protein

Structure

• [AF|P07791]

[wiki|Localization]

• inner spore coat  [Pubmed|19933362], localization depends on [protein|CEBEC9CECCF445C40D793E61A2CD23175631A473|safA]  [Pubmed|22171814]

Expression and Regulation

Operons

• genes: [gene|FA8D9AA0968365522DEFC05B277123FC96482044|cotD]

  description: [Pubmed|22383849]

  regulation

  • expressed late during sporulation in the mother cell ([protein|search|SigK], [wiki|SpoIIID], [protein|search|GerE]) [Pubmed|15383836,15699190,7966271]

  regulatory mechanism

  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|7966271], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]
  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]: activation, [Pubmed|15383836], in [regulon|protein:19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE regulon]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15699190], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]

• genes: [gene|4D286C045A43FBF0F6E93E9E46AD7F25E35ADA7C|mrfA]-[gene|3F1D78CAAA4B5057DEBB24C4D382F96DA4AA4852|mrfB]-[gene|FA8D9AA0968365522DEFC05B277123FC96482044|cotD]

  description: [Pubmed|22383849]

Biological materials

Mutant

• 1S104 ( ''cotD''::''cat''), [Pubmed|2821284], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S104&Search=1S104 BGSC]
• BKE22200 (Δ[gene|FA8D9AA0968365522DEFC05B277123FC96482044|cotD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22200 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATAAAATCCCCTTTCC,  downstream forward: _UP4_TAGTATGGAAAACGTGCAGC
• BKK22200 (Δ[gene|FA8D9AA0968365522DEFC05B277123FC96482044|cotD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22200 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATAAAATCCCCTTTCC,  downstream forward: _UP4_TAGTATGGAAAACGTGCAGC

References

Reviews

• 23202530,35638784

Original Publications

• 19933362,1518043,2821284,2492118,1691789,14651647,15699190,7966271,22171814,15383836