clsA

168

cardiolipin synthase, major enzyme

locus: BSU_36590

Molecular weight: 55.70 kDa

pI: 9.55

Protein length: 482 aa

Gene length: 1449 bp

function: biosynthesis of phospholipids

product: cardiolipin synthase, major enzyme

essential: no

synonyms: clsA, ywnE

List of homologs in different organisms, belongs to COG1502 (Galperin et al., 2021)

Gene

Coordinates: 3,762,664  3,764,112

Phenotypes of a mutant

• increased conjugation of ICEBs1 [Pubmed|26833415]

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The protein

Catalyzed reaction/ biological activity

• 2 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) --> cardiolipin + glycerol (according to UniProt)

Protein family

• phospholipase D family (with [protein|B63AAF0D3277FD776944A09D2546F3E48C8716AD|ywjE] and [protein|07752EC6940F43DEBD1E9313A2A195E35EDB9F69|ywiE], according to UniProt)

[wiki|Domains]

• 2 PLD phosphodiesterase domains (aa 217-244, aa 395-422) (according to UniProt)

Structure

• [AF|P71040]

Paralogous protein(s)

• [protein|B63AAF0D3277FD776944A09D2546F3E48C8716AD|ywjE], [protein|07752EC6940F43DEBD1E9313A2A195E35EDB9F69|ywiE]

[wiki|Localization]

• cell membrane at the septum, this localization depends on two C-terminal helices [Pubmed|26708983,15743965]

Biological materials

Mutant

• BKE36590 ([gene|2079B210322F26EEC3CCF55611622FADDB1D1BC7|clsA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36590 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTTTGTAACCCCGCTCAT,  downstream forward: _UP4_TAAGATGCGTAAACCCCCGG
• BKK36590 ([gene|2079B210322F26EEC3CCF55611622FADDB1D1BC7|clsA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36590 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTTTGTAACCCCGCTCAT,  downstream forward: _UP4_TAAGATGCGTAAACCCCCGG

References

• 18820022,14973018,16514141,15743965,26708983,26833415,28376879