cheY

168

two-component response regulator, modulation of flagellar switch bias, CheY-phosphate induces a switch from clockwise to counter-clockwise rotation

locus: BSU_16330

Molecular weight: 13.17 kDa

pI: 4.75

Protein length: 120 aa

Gene length: 363 bp

function: modulation of flagellar switch bias

product: two-component response regulator

essential: no

synonyms: cheY, cheB

List of homologs in different organisms, belongs to COG2204 (Galperin et al., 2021)

Gene

Coordinates: 1,703,834  1,704,196

Phenotypes of a mutant

• not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation [Pubmed|26122431]
• defective in [category|SW.4.1.4|Swarming] motility [pubmed|35638827]

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The protein

[wiki|Domains]

• [wiki|Response regulatory domain] (aa 4-119) (according to UniProt)

Structure

• [PDB|1TMY] ([protein|2F0495C7EAB81BDB1F3181677C555537BD2A972F|cheY] from ''Thermotoga maritima'')
• [AF|P24072]

Modification

• phosphorylated by [protein|6B3B222E56BF0C95A2371CA5208B5522B44D4689|cheA] on an Asp residue
• dephosphorylated by [protein|DC9B5446484E42F46EB2B0541192D7D8B4B3AC8F|cheC] [Pubmed|23226535]

Paralogous protein(s)

• [protein|AE823A0A652509787EA032F122E59F3C3645385F|yneI]

[wiki|Localization]

• cytoplasm (according to UniProt)

Biological materials

Mutant

• 1A798 ( ''cheY''::''cat''), [Pubmed|1905718], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A798&Search=1A798 BGSC]
• DS6870 (marker-less in NCIB3610) [Pubmed|25313396]
• BKE16330 ([gene|2F0495C7EAB81BDB1F3181677C555537BD2A972F|cheY]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16330 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAATCTATCTCTCTCCTT,  downstream forward: _UP4_TTAAATTAAAGGGTGTACGA
• BKK16330 ([gene|2F0495C7EAB81BDB1F3181677C555537BD2A972F|cheY]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16330 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAATCTATCTCTCTCCTT,  downstream forward: _UP4_TTAAATTAAAGGGTGTACGA

References

Reviews

• 25313396,26122431,19494578,10094672,20122866,16224120,30718302

Original Publications

• 17908686,10196193,17850253,12920116,8369293,9194713,14651647,9657996,8157612,15175317,14749334,20080618,17675386,21515776,24386445,35638827