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  • Description: hydrophobic toxin; interferes with cell envelope biosynthesis, causes membrane invaginations together with delocalization of the cell wall synthesis machinery, triggers autolysis, and inhibits protein biosynthesis

Gene name bsrG
Essential no
Product class I toxin
Function unknown
MW, pI
Gene length, protein length 294 bp, 38 aa
Immediate neighbours yolA, SR4
Get the DNA and protein
Genetic context
File:BsrG context.gif
This image was kindly provided by SubtiList

Categories containing this gene/protein

toxins, antitoxins and immunity against toxins, SPß prophage, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag:

Phenotypes of a mutant

Database entries

  • BsubCyc: ?
  • DBTBS entry: no entry
  • SubtiList entry: no entry

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • BsubCyc: ?
  • Structure:
  • UniProt:
  • KEGG entry:
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expression increases at the onset of the stationary phase PubMed
    • the mRNA is destabilized upon interaction with the antitoxin RNA SR4 PubMed
    • the mRNA is degraded by Rnc, Rny and Rnr PubMed
  • Regulatory mechanism:
    • the mRNA is degraded by Rnc, Rny and Rnr, degradation is enhanced at higher temperature PubMed
  • Additional information:
    • half-life of the mRNA: 15 min PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:

Labs working on this gene/protein

Sabine Brantl, Jena, Germany

Your additional remarks


Natalie Jahn, Sabine Brantl, Henrik Strahl
Against the mainstream: the membrane-associated type I toxin BsrG from Bacillus subtilis interferes with cell envelope biosynthesis without increasing membrane permeability.
Mol Microbiol: 2015, 98(4);651-66
[PubMed:26234942] [] [DOI] (I p)

Natalie Jahn, Sabine Brantl
One antitoxin--two functions: SR4 controls toxin mRNA decay and translation.
Nucleic Acids Res: 2013, 41(21);9870-80
[PubMed:23969414] [] [DOI] (I p)

Natalie Jahn, Heike Preis, Christoph Wiedemann, Sabine Brantl
BsrG/SR4 from Bacillus subtilis--the first temperature-dependent type I toxin-antitoxin system.
Mol Microbiol: 2012, 83(3);579-98
[PubMed:22229825] [] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [] [DOI] (I p)

Shinichi Saito, Hiroshi Kakeshita, Kouji Nakamura
Novel small RNA-encoding genes in the intergenic regions of Bacillus subtilis.
Gene: 2009, 428(1-2);2-8
[PubMed:18948176] [] [DOI] (P p)