bpsA

168

type III polyketide synthase

locus: BSU_22050

Molecular weight: 40.55 kDa

pI: 7.96

Protein length: 365 aa

Gene length: 1098 bp

function: polyketide synthesis

product: type III polyketide synthase

essential: no

ec: 2.3.1.74

synonyms: bpsA, bcsA

List of homologs in different organisms, belongs to COG3424 (Galperin et al., 2021)

Gene

Coordinates: 2,316,956  2,318,053

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The protein

Catalyzed reaction/ biological activity

• synthesis of triketide pyrones from long-chain fatty acyl CoA thioesters as starter substrates and malonyl-CoA as an extender substrate [Pubmed|19465653]
• 4-coumaroyl-CoA + 2 H⁺ + 3 malonyl-CoA --> 2',4,4',6'-tetrahydroxychalcone + 3 CO₂ + 4 CoA (according to UniProt)

Protein family

• [wiki|thiolase-like superfamily] (according to UniProt)

Structure

• [PDB|4JAO] (from Mycobacterium tuberculosis, 34% identity) [pubmed|23615910]
• [AF|P54157]

Expression and Regulation

Operons

• genes: [gene|7D90F7AF49BAD6A95731BF805991DEBBBCAFA46C|bpsA]-[gene|6BAF5763249305F27326F941A34956396B3F8C99|bpsB]

  description: [Pubmed|19465653]

Biological materials

Mutant

• MGNA-A875 (bcsA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/875 NBRP B. subtilis, Japan]
• BKE22050 (''[gene|7D90F7AF49BAD6A95731BF805991DEBBBCAFA46C|bpsA]''::''erm'', available in the BGSC and in [wiki|Jörg Stülke]'s lab) [pubmed|28189581]
• GP1820 (''[gene|7D90F7AF49BAD6A95731BF805991DEBBBCAFA46C|bpsA]''::''erm'', available in [wiki|Jörg Stülke]'s lab)
• BKE22050 ([gene|7D90F7AF49BAD6A95731BF805991DEBBBCAFA46C|bpsA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22050 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCGATCACCTCTTTGCA,  downstream forward: _UP4_AGCTGGGAAAAGGGGGCCTG
• BKK22050 ([gene|7D90F7AF49BAD6A95731BF805991DEBBBCAFA46C|bpsA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22050 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCGATCACCTCTTTGCA,  downstream forward: _UP4_AGCTGGGAAAAGGGGGCCTG

References

• 19465653,23615910