bglH

168

phospho-beta-glucosidase

locus: BSU_39260

Molecular weight: 53.12 kDa

pI: 4.96

Protein length: 469 aa

Gene length: 1410 bp

function: salicin utilization

product: phospho-beta-glucosidase

essential: no

ec: 3.2.1.86

synonyms: bglH

List of homologs in different organisms, belongs to COG2723 (Galperin et al., 2021)

Gene

Coordinates: 4,032,346  4,033,755

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The protein

Catalyzed reaction/ biological activity

• 6-phospho-β-D-glucosyl-(1→4)-D-glucose + H₂O --> D-glucose + D-glucose 6-phosphate (according to UniProt)

Protein family

• [wiki|glycosyl hydrolase 1 family] (according to UniProt)

Structure

• [PDB|4IPL] (from ''Streptococcus pneumoniae'', 60% identity) [Pubmed|23580646]
• [AF|P40740]

Expression and Regulation

Operons

• genes: [gene|35E6C81BE481C3E57CF47B781580368C3AC15D83|bglP]-[gene|8D98212930CF6613D332BF99452B231CB6426E93|bglH]-[gene|5A4CDE1CFE319E4471A15702ED1632C96270528D|yxiE]

  description: [Pubmed|7883710]

  regulation

  • induced by salicin ([protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT]) [Pubmed|7883710]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA] binding site overlaps -35 region) [Pubmed|7559347], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
  • [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT]: anti-termination, via [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT]-dependent [wiki|RNA switch], lack of [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT]-dependent antitermination in the presence of gucose due to the requirement of [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT] to be phosphorylated by [protein|771F205F818A755A661B8E1C95365A4F6AEB05C7|hprK], in [regulon|protein:FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7559347], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

  additional information

  • An [wiki|ncRNA] is predicted for '[protein|35E6C81BE481C3E57CF47B781580368C3AC15D83|bglP]' [PubMed|20525796]

Biological materials

Mutant

• BKE39260 ([gene|8D98212930CF6613D332BF99452B231CB6426E93|bglH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39260 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTGATCACCCCATTAG,  downstream forward: _UP4_TGACAATTTTTTGAAAACTC
• BKK39260 ([gene|8D98212930CF6613D332BF99452B231CB6426E93|bglH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39260 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTGATCACCCCATTAG,  downstream forward: _UP4_TGACAATTTTTTGAAAACTC

References

• 14652714,16672620,15139916,7883710,7559347,26772920,23580646